葛根芩连汤含药血清对IR-HepG2细胞糖代谢调控关系分析

目的:探索葛根芩连汤含药血清对胰岛素抵抗(IR)的HepG2(IR-HepG2)细胞糖代谢的调控关系。方法:将正常的HepG2细胞作为空白组,IR-HepG2细胞分为模型组,非诺贝特组,葛根芩连汤低、中、高剂量组,以葡萄糖消耗量为药效指标,采用色谱-质谱联用技术,对IR-HepG2细胞及给药后的IR-HepG2细胞内代谢产物进行分析,经过Mass Profiler Professional(MPP)软件对数据进行分析,得出生物标志物,验证之后,研究呈剂量依赖的生物标志物对糖耗量的关系。结果:发现葛根芩连汤含药血清能提高IR-HepG2细胞的糖耗量,其调节机制可能和文中6个生物标记物有关,已确定其中3个生物标志物是存在剂量依赖性且对糖代谢具有调控作用。结论:葛根芩连汤含药血清可能通过调节IR-HepG2细胞色氨酸,泛酸和腺嘌呤的量达到提高糖代谢的作用。

Effect of Gegen Qinlian Tang Serum in Regulating Glucose Metabolism of IR-HepG2 Cells

Objective: To explore the regulatory effect of Gegen Qinlian Tang serum on the glucose metabolism of insulin resistance (IR) HepG2 (IR-HepG2). Method: Normal HepG2 cells were taken as blank control group. IR-HepG2 cells were divided into model group, fenofibrate group, low and high-dose Gegen Qinlian Tang group. With glucose consumption as the pharmacodynamic index, LC-MS was used for the analysis of metabolites of IR-HepG2 cells and IR-HepG2 cells. Mass Profiler Professional (MPP) software was used to analyze the data to obtain biomarkers; after validation, the relationship between glucose consumption and biomarkers was studied. Result: Three biomarkers were found to be dose dependent and could regulate glucose metabolism. Conclusion: Gegen Qinlian Tang-containing serum could regulate the content of tryptophan, pantothenic acid and adenine in IR-HepG2 cell to reduce sugar metabolism.