首页 | 本学科首页   官方微博 | 高级检索  
     

葛根芩连汤含药血清对IR-HepG2细胞糖代谢调控关系分析
引用本文:陈瑶,徐国良,李冰涛,姜丽,盛译萱,周子妍,张启云. 葛根芩连汤含药血清对IR-HepG2细胞糖代谢调控关系分析[J]. 中国实验方剂学杂志, 2018, 24(10): 156-160
作者姓名:陈瑶  徐国良  李冰涛  姜丽  盛译萱  周子妍  张启云
作者单位:江西中医药大学
基金项目:国家自然科学基金项目(81403158,81560744);江西省卫生厅基金项目(2012A161);江西省教育厅基金项目(GJJ13586)
摘    要:目的:探索葛根芩连汤含药血清对胰岛素抵抗(IR)的HepG2(IR-HepG2)细胞糖代谢的调控关系。方法:将正常的HepG2细胞作为空白组,IR-HepG2细胞分为模型组,非诺贝特组,葛根芩连汤低、中、高剂量组,以葡萄糖消耗量为药效指标,采用色谱-质谱联用技术,对IR-HepG2细胞及给药后的IR-HepG2细胞内代谢产物进行分析,经过Mass Profiler Professional(MPP)软件对数据进行分析,得出生物标志物,验证之后,研究呈剂量依赖的生物标志物对糖耗量的关系。结果:发现葛根芩连汤含药血清能提高IR-HepG2细胞的糖耗量,其调节机制可能和文中6个生物标记物有关,已确定其中3个生物标志物是存在剂量依赖性且对糖代谢具有调控作用。结论:葛根芩连汤含药血清可能通过调节IR-HepG2细胞色氨酸,泛酸和腺嘌呤的量达到提高糖代谢的作用。

关 键 词:葛根芩连汤含药血清  HepG2细胞  细胞代谢组学  生物标志物  调控关系
收稿时间:2017-11-07

Effect of Gegen Qinlian Tang Serum in Regulating Glucose Metabolism of IR-HepG2 Cells
CHEN Yao,XU Guo-liang,LI Bing-tao,JIANG Li,SHENG Yi-xuan,ZHOU Zi-yan and ZHANG Qi-yun. Effect of Gegen Qinlian Tang Serum in Regulating Glucose Metabolism of IR-HepG2 Cells[J]. China Journal of Experimental Traditional Medical Formulae, 2018, 24(10): 156-160
Authors:CHEN Yao  XU Guo-liang  LI Bing-tao  JIANG Li  SHENG Yi-xuan  ZHOU Zi-yan  ZHANG Qi-yun
Affiliation:Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China,Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China,Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China,Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China,Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China,Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China and Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
Abstract:Objective: To explore the regulatory effect of Gegen Qinlian Tang serum on the glucose metabolism of insulin resistance (IR) HepG2 (IR-HepG2). Method: Normal HepG2 cells were taken as blank control group. IR-HepG2 cells were divided into model group, fenofibrate group, low and high-dose Gegen Qinlian Tang group. With glucose consumption as the pharmacodynamic index, LC-MS was used for the analysis of metabolites of IR-HepG2 cells and IR-HepG2 cells. Mass Profiler Professional (MPP) software was used to analyze the data to obtain biomarkers; after validation, the relationship between glucose consumption and biomarkers was studied. Result: Three biomarkers were found to be dose dependent and could regulate glucose metabolism. Conclusion: Gegen Qinlian Tang-containing serum could regulate the content of tryptophan, pantothenic acid and adenine in IR-HepG2 cell to reduce sugar metabolism.
Keywords:Gegen Qinlian Tang serum  HepG2 cell  cell metabolomics  biomarker  regulatory relationship
本文献已被 CNKI 等数据库收录!
点击此处可从《中国实验方剂学杂志》浏览原始摘要信息
点击此处可从《中国实验方剂学杂志》下载全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号