基于外翻肠囊模型分析玉簪花总黄酮提取物及其复合粒子的肠吸收特性

目的:考察玉簪花总黄酮提取物及其复合粒子中有效成分的肠吸收特性。方法:采用离体外翻肠囊模型,选择十二指肠、空肠、回肠、结肠为研究肠段,以表观渗透系数为评价参数,考察玉簪花总黄酮提取物及其复合粒子中有效成分山柰酚-3-O-槐糖苷和山柰酚-3-O-芸香糖苷在不同肠段、不同浓度下的肠吸收特性。结果:在不同药物质量浓度下,山柰酚-3-O-槐糖苷及山柰酚-3-O-芸香糖苷在各肠段均有吸收;在同一质量浓度条件下,二者均以空肠的肠吸收最优。结论:玉簪花总黄酮复合粒子改变了山柰酚-3-O-槐糖苷及山柰酚-3-O-芸香糖苷的肠吸收特性,二者在结肠的吸收显著增加。玉簪花总黄酮复合粒子可显著提高该有效部位在结肠部位的吸收。

Analysis on Intestinal Absorption Properties of Total Flavonoids Extract of Hosta plantaginea Dried Flowers and Its Composite Particles Based on Everted Gut Sac Model

Objective: To investigate intestinal absorption properties of active ingredients in total flavonoids extract of Hosta plantaginea dried flowers and its composite particles. Method: Everted gut sac model was employed.The duodenum, jejunum, ileum and colon of rats were selected as intestinal segment for research.The apparent permeability coefficient(P_app) was used to evaluate the intestinal absorption characteristics of active ingredients from total flavonoids extract of H. plantaginea dried flowers and its composite particles in different intestinal segments and different concentrations. Result: Under different drug concentrations, kaempferol-3-O-sophoroside and kaempferol-3-O-rutinoside were absorbed in all intestinal segments;at the same concentration, their absorption in the jejunum were the best. Conclusion: The composite particles of total flavonoids extract of H. plantaginea dried flowers changes the intestinal absorption characteristics of kaempferol-3-O-sophoroside and kaempferol-3-O-rutinoside, and it can notably enhance their absorption in the colon.The composite particles can significantly increase the absorption of total flavonoids extract of H. plantaginea dried flowers in the colon.