| 北沙参多糖的分离、纯化及其体外免疫活性考察 |
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| 引用本文： | 杜宝香,相美容,付业佩,张静,蒋海强,容蓉. 北沙参多糖的分离、纯化及其体外免疫活性考察[J]. 中国实验方剂学杂志, 2018, 24(11): 27-31 |
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| 作者姓名： | 杜宝香 相美容 付业佩 张静 蒋海强 容蓉 |
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| 作者单位： | 山东中医药大学药学院实验中心 |
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| 基金项目： | 国家“重大新药创制”科技重大专项（2017ZX09101003-003-004）；山东省重点研发计划项目（2017CXGC1309） |
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| 摘 要： | 目的:分离、纯化北沙参多糖(Glehniae Radix polysaccharides,GRP)并对各纯化组分(GRP-1,GRP-2,GRP-3)进行结构表征及体外免疫活性研究。方法:采用水提醇沉法获得GRP,Sevage法除蛋白,活性炭脱色素,DEAE纤维素DE-52柱色谱法及葡聚糖凝胶G-75(Sephadex G-75)柱色谱法分离与纯化。采用紫外分光光度法(UV)及1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化-HPLC对北沙参多糖的含量及其单糖组成进行分析,采用傅里叶红外光谱仪(IR)及多角度激光散射仪对GRP-1进行结构表征及相对分子质量测定,并考察北沙参多糖体外对小鼠脾淋巴细胞增殖活性的影响。结果:GRP经柱色谱分离可获得GRP-1,GRP-2,GRP-3共3种多糖组分,UV测得三者的多糖质量分数分别为96.16%,48.64%和89.73%。其中,GRP-1为具有相对均一分子质量的多糖组分,相对分子质量23.01 k Da,由甘露糖-葡萄糖醛酸-鼠李糖-葡萄糖-半乳糖-阿拉伯糖组成,摩尔比为81.86∶0.12∶0.17∶1 259.7∶0.54∶0.33,红外光谱表明其结构中存在α型糖苷键。体外免疫活性结果表明GRP-1组分对体外脾脏淋巴细胞的增殖具有较好的促进作用,此外GRP-2及GRP-3对脾脏淋巴细胞的增殖也具有一定的推动作用。结论:GRP-1是由多种单糖组成的相对均一的多糖组分,并具有较好的体外免疫活性。
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| 关 键 词： | 北沙参 多糖 分离 纯化 免疫活性 抗肿瘤 红外光谱 |
| 收稿时间： | 2017-10-13 |
Investigation of Isolation,Purification,Structural Identification and in Vitro Immunological Function of Polysaccharides in Glehniae Radix |
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| DU Bao-xiang,XIANG Mei-rong,FU Ye-pei,ZHANG Jing,JIANG Hai-qiang and RONG Rong. Investigation of Isolation,Purification,Structural Identification and in Vitro Immunological Function of Polysaccharides in Glehniae Radix[J]. China Journal of Experimental Traditional Medical Formulae, 2018, 24(11): 27-31 |
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| Authors: | DU Bao-xiang XIANG Mei-rong FU Ye-pei ZHANG Jing JIANG Hai-qiang RONG Rong |
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| Affiliation: | Experimental Center, School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China,Experimental Center, School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China,Experimental Center, School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China,Experimental Center, School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China,Experimental Center, School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China and Experimental Center, School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China |
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| Abstract: | |
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| Keywords: | Glehniae Radix polysaccharides isolation purification immune activity anti-tumor infrared spectroscopy |
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