三种中药药效成分抗支气管哮喘变应性炎症的实验研究

目的 :通过豚鼠哮喘动物模型研究三种中药有效成分 (银杏内酯、黄芩甙、川芎嗪 )雾化吸入抗哮喘气道变应性炎症的作用。方法 :以卵蛋白致敏豚鼠为动物模型 ,雾化吸入予以银杏内酯、川芎嗪、黄芩甙。观察治疗前后各组豚鼠的血嗜酸性粒细胞数、血嗜酸细胞阳离子蛋白 (ECP)以及支气管肺泡灌洗液 (BALF)中炎性细胞和嗜酸性粒细胞、嗜酸细胞阳离子蛋白改变。结果 :同模型组相比 ,银杏内酯、黄芩甙、川芎嗪均可不同程度地抑制哮喘豚鼠外周血嗜酸细胞计数 ;黄芩甙和川芎嗪对BALF中的嗜酸细胞及炎性细胞总数均有抑制作用 ,而银杏内酯仅抑制BALF中嗜酸细胞聚集。各治疗组均未显示预防哮喘发作的作用 ,各组的ECP也无显著性差异。结论 :银杏内酯、黄芩甙、川芎嗪通过抑制哮喘动物外周血及BALF中嗜酸细胞聚集而具有一定的抗哮喘气道变应性炎症的作用

Experimental Study of the Effect of Components of Three Traditional Chinese Medcines on Airway Allegic Inflammation in Guinea Pigs with Bronchial Asthma

Objective:To observe the effect of ginkgolide,baiclin and linguitrazin on airway allergic inflammation in Guinea pigs with asthma.Methods:Eosinophil cells count in blood and bronchial alveolar lavage fluid(BALF),as well as total cells count in BALF were measured by traditional methods.Eosinophil cation protein (ECP) level in blood and BALF was detected by radioimmunoassay.Results:Treatments with ginkgolide,baicalin and linguitrazin could significantly inhibit blood eosinophil gathering in asthma animals comparing with the untreated animals.Baicalin and linguitrazin also reduced eosinophil and total cells count in BALF,while ginkgolide only could inhibit the total cells count.All components of TCM concerned had no protection effect on asthma attack.No difference was shown on ECP levels among all groups.Conclusion:Ginkgolide,baicalin and linguitrazin could inhibit the AAI of asthma animals by reducing eosinophol gathering in blood and BALF.There was no protection effect found suggested that the compound of such components might be effective in treating and preventing asthma attack.