| 实验性大鼠肾草酸钙结石造模方法筛选研究 |
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| 引用本文： | 赖海标,刘朝晖,吴松,欧秀华,顾向明,王书芹,高玉桥,林慧,汪芸,戴卫波,范文昌,丘兰英,林家乐,梅全喜.实验性大鼠肾草酸钙结石造模方法筛选研究[J].中国实验方剂学杂志,2010,16(14):135-138. |
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| 作者姓名： | 赖海标 刘朝晖 吴松 欧秀华 顾向明 王书芹 高玉桥 林慧 汪芸 戴卫波 范文昌 丘兰英 林家乐 梅全喜 |
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| 作者单位： | 1. 中山市中医院,广东中山,528400
2. 广州中医药大学,广州,510006 |
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| 基金项目： | 中山市科技计划项目，广东省中医药局科技基金项目 |
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| 摘 要： | 目的:筛选一种方法简便、稳定、成石效果好的实验性大鼠肾草酸钙结石造模方法。方法:采用几种目前常用的实验性大鼠肾草酸钙结石造模方法(造模Ⅰ组:1%乙二醇饮水+2%氯化铵每天ig 2 mL/只;造模Ⅱ组:1.25%乙二醇+1%氯化铵,ig 20 mL.kg-1.d-1;造模Ⅲ组:每只每天饮用1.25%乙二醇+1%氯化铵10 mL,水30 mL;造模Ⅳ组:1%乙二醇+1%氯化铵自由饮用)进行造模,并进行各组尿液、血液和肾组织中的多项生化指标【尿素氮(BUN)、肌酐(Cr)、尿酸(UA)、无机磷(IP)、钙(Ca2+)、草酸(OX)】检测以及肾脏病理标本观察,综合评价造模效果。结果:造模Ⅰ组的24 h尿液中的BUN,Cr,UA,IP,Ca2+,OX均高于正常对照组(Ⅴ组),且统计学上有极显著性差异(P0.01);血液中BUN,Cr,UA,IP,Ca2+均高于正常对照组,且统计学上有极显著性差异(P0.01);肾组织中Ca2+和OX均高于正常对照组,且统计学上有极显著性差异(P0.01);肾组织可见大量草酸钙结晶沉积。造模Ⅱ组和造模Ⅲ组的多项生化指标结果显示大鼠的肾功能下降,但肾组织中未见结晶体,仅发现肾间质血管充血。造模Ⅳ组大鼠全部死亡。结论:造模Ⅰ组为最佳造模方法。
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| 关 键 词： | 草酸钙 肾结石 动物模型 大鼠 |
| 收稿时间： | 2010/5/17 0:00:00 |
Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats |
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| LAI Hai-biao,LIU Zhao-hui,WU Song,OU Xiu-hu,GU Xiang-ming,WANG Shu-qin,GAO Yu-qiao,LIN Hui,WANG Yun,DAI Wei-bo,FAN Wen-chang,QIU Lan-ying,LIN Jia-le and MEI Quan-xi.Comparison of Experimental Renal Calculus of Calcium Oxalate in Rats[J].China Journal of Experimental Traditional Medical Formulae,2010,16(14):135-138. |
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| Authors: | LAI Hai-biao LIU Zhao-hui WU Song OU Xiu-hu GU Xiang-ming WANG Shu-qin GAO Yu-qiao LIN Hui WANG Yun DAI Wei-bo FAN Wen-chang QIU Lan-ying LIN Jia-le and MEI Quan-xi |
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| Institution: | Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Guangzhou University of Traditional Chinese Medicine, Guangzhou 510006, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China;Hospital of Traditional Chinese Medicine of Zhongshan, Zhongshan 528400, China |
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| Abstract: | |
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| Keywords: | calcium oxalate renal calculus animal model rat |
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