| 大孔树脂纯化浙贝母中总生物碱工艺优选 |
| |
| 引用本文： | 董洁,朱会敏,胡凯文,安超,韩立炜.大孔树脂纯化浙贝母中总生物碱工艺优选[J].中国实验方剂学杂志,2012,18(14):18-22. |
| |
| 作者姓名： | 董洁 朱会敏 胡凯文 安超 韩立炜 |
| |
| 作者单位： | 1. 北京中医药大学中药学院,北京,100102
2. 北京中医药大学东方医院,北京,100078 |
| |
| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09103-346);北京中医药大学复方中药制药研究创新团队项目(2011-CXTD-13) |
| |
| 摘 要： | 目的:优选大孔树脂纯化浙贝母中总生物碱的工艺.方法:通过静态、动态吸附和解吸动力学试验,筛选最佳大孔树脂型号,并优选其纯化工艺.结果:最佳纯化工艺为采用HPD100型大孔树脂,径高比1∶9,上样液pH 8.0,上样液生药质量浓度5 g·mL-1,上样体积13 BV,吸附流速1 mL· min -1,8 BV水洗除杂,用10 BV 80％乙醇洗脱,洗脱流速1 mL· min -1,收集80％乙醇洗脱液.优选的纯化工艺可使浙贝母总生物碱含量＞65％.结论:HPD100型大孔树脂对浙贝母总生物碱的富集综合性能较好,适合用于其分离纯化.
|
| 关 键 词： | 浙贝母 总生物碱 大孔树脂 纯化 |
| 收稿时间： | 2012/2/29 0:00:00 |
Optimization of Purification Process for Total Alkaloids from Fritillaria thunbergii by Macroporous Resin |
| |
| DONG Jie,ZHU Hui-min,HU Kai-wen,AN Chao and HAN Li-wei.Optimization of Purification Process for Total Alkaloids from Fritillaria thunbergii by Macroporous Resin[J].China Journal of Experimental Traditional Medical Formulae,2012,18(14):18-22. |
| |
| Authors: | DONG Jie ZHU Hui-min HU Kai-wen AN Chao and HAN Li-wei |
| |
| Institution: | School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China;Oriental Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;Oriental Hospital,Beijing University of Chinese Medicine,Beijing 100078,China;School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China |
| |
| Abstract: | Objective:To optimize purification process of total alkaloids from Fritillaria thunbergii by macroporous resin.Method:Optimum macroporous resin and its purification process was selected by static and dynamic adsorption and desorption kinetics test.Result:Optimal purification process conditions were:optimal macroporous resin type HPD100,diameter-height ratio 1∶9,the concentration of sample solution 5 g·mL-1 with pH as 8.0,maximum sample volume 13 BV,flow rate of adsorption and elution 1 mL·min-1,washed by 8 BV water and 10 BV 80% ethanol,collected eluent of 80% ethanol.Under this optimized technology,the content of total alkaloids was more than 65% by UV.Conclusion:HPD100 type macroporous resin showed good comprehensive adsorption property for enrichment of total alkaloids from F.thunbergii,and suitable for separation and purification of it. |
| |
| Keywords: | Fritillaria thunbergii total alkaloids macroporous resin purification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
|
