降压中药有效组分配伍对离体大鼠胸主动脉环的舒张作用

目的:研究天麻素、黄芩苷、川芎嗪、葛根素4种中药有效单体及其组合对大鼠胸主动脉的舒张作用,筛选出最佳组合.方法:以大鼠胸主动脉环为标本,观察单组分及组合物对去甲肾上腺素(NE)预收缩的血管舒张作用.结果:天麻素、黄芩苷、川芎嗪、葛根素单体存在浓度依赖性的舒张血管效应.正交实验结果显示天麻素(0.64 g·L-1)、黄芩苷(0.12 g·L-1)、川芎嗪(0.32 g·L-1)、葛根素(0.64 g·L-1)是最优组合,舒张率达到89.37％.结论:单组分及组合物对NE预收缩的大鼠胸主动脉均具有舒张作用,组合配伍强于单组分.

Vasodilative Action of Combinations of Hypotensive Components of Traditional Chinese Medicines on Rat Isolated Thoracic Aorta

Objective: To investigate the vasodilative action of gastrodin, baicalin, tetramethylpyrazine,puerarin and their combinations on rat thoracic aorta, and to select the best combination of hypotensive components. Method: The rat thoracic aorta was taken as specimen and the vasodilative action of single components and their combinations on rat thoracic aorta which was pre-constricted with NE was tested. Result: Gastrodin, baicalin, tetramethylpyrazine and puerarin exerted a dose-dependent vasodilative action on rat isolated thoracic aorta rings. The results of orthogonal optimization experiment showed that the best combination including gastrodin (0.64 g·L^-1), baicalin (0.12 g·L^-1), tetramethylpyrazine (0.32 g·L^-1) and puerarin (0.64 g·L^-1) presented more obvious contraction than any single component. And the vasodilatation rate was 89.37%. Conclusion: The above components and their combination have vasodilative action on the NE pre-constricted rat thoracic aorta, but the effect of latter is stronger than the former.