| 槐定碱对急性肺损伤小鼠肺组织SOD, MDA及TLR4表达的影响 |
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| 引用本文： | 杨翠萍,杨晓金,田真真,叶荷平,朱金华,付艳丽,万红娇.槐定碱对急性肺损伤小鼠肺组织SOD, MDA及TLR4表达的影响[J].中国实验方剂学杂志,2012,18(14):180-184. |
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| 作者姓名： | 杨翠萍 杨晓金 田真真 叶荷平 朱金华 付艳丽 万红娇 |
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| 作者单位： | 1. 江西中医学院,南昌,330004
2. 南昌大学医学院,南昌,330006 |
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| 基金项目： | 江西省教育厅青年科学基金项目(GJJ11190) |
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| 摘 要： | 目的:研究槐定碱对内毒素(LPS)致急性肺损伤(ALI)小鼠肺组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量及Toll样受体4(TLR4)表达的影响.方法:采用随机数字法,将60只清洁级昆明种小鼠分成6组:正常组、模型组、预防给药组、槐定碱低剂量组、槐定碱中剂量组、槐定碱高剂量组,每组10只.①正常组、②模型组、④槐定碱低剂量组、⑤槐定碱中剂量组、⑥槐定碱高剂量组:ip生理盐水0.5 mL,连续6d.③预防给药组:ip 5 mg·kg-1槐定碱,连续6d.上述②～⑥组均在末次注射生理盐水或药物后1 h ip脂多糖(LPS)9 mg· kg -1造模,造模2h后④～⑥组分别给槐定碱9,5,2.5 mg·kg-1.6h后检测肺组织匀浆中SOD活性及MDA的含量,取新鲜肺组织裂解后提取RNA,RT-PCR法检测TLR4 mRNA的表达水平.结果:与正常组(86.22 ±8.86) U·mg-1相比,LPS模型组(50.58±10.06) U·mg-1肺组织匀浆SOD活性明显下降；与模型组相比,各给药组SOD预防给药组(64.71±5.74)U·mg-1,高剂量组(56.34 ±6.88) U·mg-1,中剂量组(75.50 ±13.01)U·mg-1,低剂量组(67.02±10.19)U·mg-1]均明显升高(P＜0.05)；而肺组织中MDA含量变化趋势与SOD相反(P＜0.05)；TLR4 mRNA的表达灰度值与正常组(0.45±0.20)比较,LPS模型组(0.82±0.20)明显升高；与模型组比较,各用药组预防给药组(0.60 ±0.32)s,高剂量(0.55 ±0.19),中剂量(0.54 ±0.29),低剂量组(0.61±0.26)]均明显降低(P＜0.05).结论:槐定碱能降低ALI模型小鼠肺组织匀浆中MDA的活性,提高SOD含量,降低TLR4 mRNA的表达.
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| 关 键 词： | 槐定碱 急性肺损伤 脂多糖 超氧化物歧化酶 丙二醛 Toll样受体 |
| 收稿时间： | 2011/12/22 0:00:00 |
Effect of Sophoridine on Expression of Superoxide Dismutase,Malonaldehyde and TLR4 mRNA in Acute Lung Injury Mice Induced by Lipopolysaccharide |
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| YANG Cui-ping,YANG Xiao-jin,TIAN Zhen-zhen,YE He-ping,ZHU Jin-hu,FU Yan-li and WAN Hong-jiao.Effect of Sophoridine on Expression of Superoxide Dismutase,Malonaldehyde and TLR4 mRNA in Acute Lung Injury Mice Induced by Lipopolysaccharide[J].China Journal of Experimental Traditional Medical Formulae,2012,18(14):180-184. |
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| Authors: | YANG Cui-ping YANG Xiao-jin TIAN Zhen-zhen YE He-ping ZHU Jin-hu FU Yan-li and WAN Hong-jiao |
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| Institution: | Jiangxi University of Traditional Chinese Medicine, Nanchang 330004,China;Grade 2010 Postgraduate, Medical School of Nanchang University, Nanchang 330006,China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004,China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004,China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004,China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004,China;Jiangxi University of Traditional Chinese Medicine, Nanchang 330004,China |
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| Abstract: | |
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| Keywords: | sophoridine acute lung injury lipopolysaccharide super oxide dismutase malonaldehyde Toll-like receptor |
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