基于灰色关联分析的芒果叶提取物抗炎作用的谱效关系

目的:探讨芒果叶提取物的高效液相色谱(HPLC)图谱中主要色谱峰与抗炎药效的相关性,为表征芒果叶抗炎作用物质基础提供依据。方法:采用HPLC法建立芒果叶不同极性提取物的指纹图谱。取昆明小鼠随机分别为模型组、地塞米松阳性对照组、芒果叶提取物100 g·kg-1组,模型组给生理盐水,其余各组给予相应药物,灌胃给药,连续5 d,经二甲苯造模后,测定耳肿胀度。数据经统计分析后,采用灰色关联分析谱效关系。结果:芒果叶不同极性溶剂提取物的HPLC指纹图谱有明显差异,从HPLC指纹图谱中共提取14个能够表征药材特征的共有峰。不同极性提取物的抗炎作用存在显著差异。与模型组比较,地塞米松组具有显著抑制小鼠耳肿胀作用(P<0.05);与模型组比较,芒果叶(红象牙和台农一号)的三氯甲烷、乙酸乙酯提取物均无显著抗炎作用,其他溶剂提取物具有显著抗炎作用(P<0.05)。灰色关联分析明确了X1,X3和芒果苷对抗炎作用贡献程度最大。结论:芒果叶提取物抗炎作用的部分物质基础是芒果苷及X1,X3峰。灰色关联是研究中药谱效关系的有效手段。

Spectrum-effect Relationship of Leaf Extracts from Mangifera indica Based on Grey Relational Analysis

Objective: To investigate the spectrum-effect relationship of leaf extracts from Mangifera indica (LEMI) based on grey relational analysis. Method: High performance liquid chromatography (HPLC) was applied to establish the fingerprint chromatography of the leaf extracts extracted with different polar solvent. The anti-inflammatory effects of the extracts were observed via mice auricular swelling model induced by dimethylbenzene. Kunming mice were randomly divided into model group, dexamethasone positive group and LEMI groups. Model group was administered orally physiological saline;other groups were given corresponding drug for 5 days. Then ear swelling was determined. Then the grey relational analysis was used to investigate its spectrum-effect relationship. Result: There were significantly differences in the HPLC fingerprints and anti-inflammatory activities obtained from varied extracts. Compared with the model group, dexamethasone group had significantly inhibited the ear edema in mice (P<0.05). Chloroform extract and ethyl acetate LEMI from Red-ivory and Tainong-1 showed no significant anti-inflammatory effect, but other solvent LEMI had significant anti-inflammatory effect (P<0.05). 14 common peaks were detected in HPLC chromatography, which were used for the identification of herb products. It was found that the main anti-inflammatory effect was mostly contributed to X₁, X₃and mangiferin after grey relational analysis. Conclusion: The material basis of anti-inflammatory effect of LEMI were mangiferin, X₁ and X₃.Grey relational analysis was an effective and useful method to evaluate spectrum-effect relationship of Chinese herbs.