HPLC测定不同来源川楝子中阿魏酸的含量

目的: 建立川楝子药材中阿魏酸的含量测定方法,并对不同来源商品药材进行测定。方法: 采用高效液相色谱法,色谱柱为Ultrasphere^TM-ODS柱(4.6 mm×250 mm,5 μm),流动相乙腈-0.1%磷酸溶液(15∶85),流速1.0 mL ·min^-1,检测波长322 nm,柱温25 ℃。结果: 阿魏酸进样量在0.010~0.160 μg线性关系良好(r=0.999 9),平均回收率(n=9)为97.88%,RSD 0.68%。不同来源药材中阿魏酸的含量在0.006 7~0.027 9 mg ·g^-1。结论: 不同来源的川楝子药材中阿魏酸含量差异较大。该方法可作为评价川楝子质量的指标之一。

Determination of Ferulic Acid in Fructus Toosendan from Different Origins by HPLC

Objective: To develop an HPLC method to determine the contents of ferulic acid in Fructus Toosendan from different origins.Method: The HPLC analysis was performed on a UltrasphereTM-ODS column(4.6 mm×250 mm,5 μm),and the mobile phase consisted of acetonitrile-0.1% phosphoric acid solution(15∶85) at a flow rate of 1.0 mL·min-1.The detective wavelength was at 322 nm and the column temperature was 25 ℃.Result: The calibration curve was linear in the range of 0.010-0.160 μg(r=0.999 9),and the average recovery(n=9) was 97.88%.The contents of ferulic acid in Fructus Toosendan from different origins was in the rage of 0.006 7-0.027 9 mg·g-1.Conclusion: The content of ferulic acid in Fructus Toosendan samples from different origins is quite different.The method reported in this study is suitable for the quality control of Fructus Toosendan.