HPLC测定头花蓼及制剂热淋清颗粒中没食子酸的含量

目的:建立头花蓼及热淋清颗粒中没食子酸的含量测定方法.方法:采用反相高效液相色谱法,DiamonsilTM(钻石)C18柱(4.6 mm×250 mm,5μm),流动相甲醇-水-N,N-二甲基甲酰胺-冰醋酸(1∶95∶3∶1),流速1.0 mL· min-1,检测波长272 nm,柱温25℃.结果:没食子酸在0.139 6-1.3960μg线性良好(r =0.999 97).头花蓼平均回收率99.35％,RSD为2.61％,热淋清颗粒平均回收率97.86％,RSD为0.73％.结论:该法重复性好,专属性强,可用于控制头花蓼药材及其单方制剂热淋清颗粒的质量.为头花蓼药材规模化种植提供科学依据.

Determ Ina Tion of Gallic Acid in Polygonum Capita Tum and Relinqing Granules by HPLC

Objective: To establish an HPLC method for determination of the contents of gallic acid in Polygonum capitatum and Relinqing granules.Method: The reserved HPLC method was achieved.Diamonsil C18 colunm(4.6 mm×250 mm,5 μm) with MeOH-H2O-DMF-HAc(1∶ 95∶ 3∶ 1) as amobile phase at flow rate of 1.0 mL·min-1and detection wavelength was at 272 nm and the column temperature was at 25 ℃.Result: The calibration curve was linear in the range of 0.139 6-1.396 0 μg(r=0.999 97).The average recovery rate of gallic acid in P.capitatum was 99.35%(RSD 2.61%),and was 99.35%(RSD 0.73%) in Relinqing granules.Conclusion: The method is reliable,specific and with good repeatability.It can be used for the quality control of P.capitatum and Relinqing granules and provide a science bases for the planting of polygonum cap itatum.