宣痹凝胶贴膏剂中龙胆苦苷、柚皮苷、原苏木素B含量测定

目的:建立宣痹凝胶膏剂中龙胆苦苷、柚皮苷、原苏木素B含量测定方法.方法:采用高效液相色谱法,MerckRP-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.1％磷酸梯度洗脱(0～ 33 min,11％乙腈;33～36 min,11％乙腈～20％乙腈;36～67 min,20％乙腈),柱温20℃,流速0.6 mL· min-1,检测波长283 nm,进样量10 μL.结果:龙胆苦苷、柚皮苷、原苏木素B分别在0.014 9 ～0.745 0 μg,0.007 1 ～0.355 0 μg,0.002 2 ～0.107 5 μg呈良好的线性关系,平均回收率分别为98.87％ (RSD 2.19％),103.51％ (RSD 0.94％),99.58％( RSD 2.49％).结论:方法操作简便,准确,重复性好,可作为宣痹凝胶膏剂的质量控制.

Determination for Content of Gentiopicroside, Naringin and Protosappanin B from Xuanbi Gel Plaster

Objective:To establish content determination method for gentiopicroside,naringin and protosappanin B from Xuanbi gel plaster.Method:HPLC was used with Merck RP-C18 column(4.6 mm×250 mm,5 μm),gradient eluted with mobile phase of 0.1% phosphoric acid-acetonitrile(0-33 min,11% acetonitrile;33-36 min,11% acetonitrile-20% acetonitrile;36-67 min,20% acetonitrile),column temperature was 20 ℃,flow rate was 0.6 mL·min-1,detection wavelength 283 nm,injection volume was 10 μL.Result:The linear range of gentiopicroside,naringin and protosappanin B were 0.014 9-0.745 μg,0.007 1-0.355 μg,0.002 2-0.107 5 μg,respectively.Average recovery of these ingredients were 98.87%(RSD 2.19%),103.51%(RSD 0.94%),99.58%(RSD 2.49%),respectively.Conclusion:This established method was simple,accurate and repeatable,it could be used as quality control method for Xuanbi gel plaster.