马齿苋不同提取物对D-半乳糖致衰老小鼠的抗疲劳作用

目的:研究马齿苋不同提取物对D-半乳糖致衰老小鼠抗疲劳能力的影响,并探讨其机制.方法:取小鼠随机分为正常组、模型组、马齿苋正丁醇提取物(0.5,0.25 g·kg-1)组、马齿苋乙醇提取物(1,0.5 g·kg-1)组.除正常组sc生理盐水外,其他各组均sc D-半乳糖(1 g·kg-1)42 d制备衰老小鼠模型.用疲劳仪测定小鼠的抗疲劳能力,并测定脑组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性.结果:马齿苋正丁醇提取物(0.5 g·kg-1)和乙醇提取物(1 g·kg-1)组均可提高衰老小鼠的抗疲劳能力,马齿苋正丁醇提取物(0.5,0.25 g·kg-1)可降低脑组织MDA含量和提高SOD活性,马齿苋乙醇提取物(1 g·kg-1)可降低脑组织MDA含量.结论:马齿苋正丁醇提取物和乙醇提取物可提高衰老小鼠的抗疲劳能力,其机制可能与提高SOD活性降低MDA含量有关.马齿苋正丁醇提取物作用好于乙醇提取物.

Anti-fatigue Action of Different Extracts from Portulaca oleracea in Senile Mice by D-Gal

Objective: To study the anti-fatigue action of different extracts from Portulaca oleracea in senile mice by D-gal, and its mechanisms. Method: The mice were randomly divided into 4 groups:control group, model group, the n-butanol extracts from P. oleracea(0.5, 0.25 g·kg^-1) groups and the ethanol extracts from P. oleracea(1,0.5 g·kg^-1) groups. The control group is given hypodermic injection of isotonic NaCl in the transverse part while the other groups successively administered by hypodermic injection of D-gal(1 g·kg^-1). It is made a model of senile mice after 42 d continuation. To measure anti-fatigue performances with the Fatigue Instrument. To measure malondialdehyde(MDA) contents and superoxide dismutase(SOD) activity in brain tissue of senile mice. Result: The n-butanol extract from P. oleracea (0.5 g·kg^-1) and the ethanol extract from P. oleracea(1 g·kg^-1) could improve anti-fatigue performances.The n-butanol extracts from P. oleracea(0.5, 0.25 g·kg^-1) could decrease MDA contents and SOD activity in brain tissue. The ethanol extract from P. oleracea (1 g·kg^-1) could decrease MDA contents n brain tissue. Conclusion: The n-butanol extract from P. oleracea and the ethanol extract from P. oleracea can improve anti-fatigue performances. Its mechanism maybe related by improve SOD activity and decrease MDA contents. The n-butanol extract from P. oleracea is better than the ethanol extract from P. oleracea.