戒毒瘾丸中罗布麻叶提取工艺优选

目的:优选戒毒瘾丸中罗布麻叶的提取工艺.方法:以镇痛药效反应、金丝桃苷含量为指标,采用单因素试验筛选提取溶媒；以干浸膏得量和金丝桃苷含量为综合评价指标,通过正交试验考察料液比、提取时间和提取次数对罗布麻叶提取工艺的影响,利用HPLC测定金丝桃苷含量.结果:最佳提取工艺为加12倍量50％乙醇提取3次,每次1h.结论:优选的工艺稳定有效,为戒毒瘾丸的深入研究提供参考.

Optimiztion of Extraction Technology of Apocynum venetum in Jieduyin Pills

Objective: To optimize extraction technology of Apocynum venetum in Jieduyin pills. Method: With the content of hyperoside and analgesic efficacy reaction as indexes,extraction solvent was selected by single-factor test;With the content of hyperoside and dry extract yield as comprehensive index,effect of the amount of solvent,extraction time and frequencies on extraction technology was investigated by orthogonal test.the content of hyperoside was determined by HPLC. Result: Optimum extraction technology was as following:extracted 3 times with 12 times the amount of 50% ethanol,1 h each time. Conclusion: This optimized extraction technology was stable and effective,which could lay foundation for further study of Jieduyin pills.