工程牛黄组方牛黄降压丸与原方剂对SHR血压影响的比较研究

目的:评价工程牛黄组方的牛黄降压丸的降压作用,与原方剂进行疗效比较,为替代原组方中人工牛黄提供药效学依据.方法:采用自发性高血压大鼠(SHR)进行降压试验观察,分为模型对照组、原牛黄降压丸组和阳性药对照组,以及新组方牛黄降压丸80％,60％,30％,10％替代组.分别灌胃给予各受试药(给药剂量均为1.76 g·kg-1),连续给药14 d,采用ALC-NIBP无创血压测定分析系统,分别测定给药0,5h,6,10,14 d,停药后3,7d的血压情况.结果:给药5h后,80％工程牛黄替代组出现明显降血压作用收缩压(SBP,139.25-19.24) mmHg,舒张压(DBP,05.69±19.18)mmHg],与对照组SBP(187.2±19.24) mmHg,DBP(123.76±18.43) mmHg]比较,具有统计学差异(P＜0.05).80％和60％工程牛黄替代组均于给药6d表现出明显的降血压作用,与模型对照组比较SBP具有统计学差异(P＜0.01),于给药6d后各组降低DBP效果趋于稳定,以80％和60％工程牛黄替代组最为明显.停药后各受试组大鼠仍表现出明显的降压作用,以80％工程牛黄替代组降压效果最佳.80％和60％工程牛黄替代组具有明显的减慢心率的作用,此作用与工程牛黄含量比例成正比,而原组方未见该作用.结论:工程牛黄替代品的牛黄降压丸组方的降压作用优于原组方,具有用量少、起效快、作用强且作用持久的优点.

Comparative Study between Effect of Engineering Niuhuang Jiangya Capsule and Artificial Niuhuang Jiangya Capsule on Blood Pressure in Spontaneously Hypertension Rats

Objective: To compare the effect of engineering Niuhuang Jiangya capsule (ENJC) and artificial Niuhuang Jiangya capsule (ANJC) on blood pressure in spontaneously hypertension rats (SHR). Method: SHR were randomly divided into 7 groups in accordance with the systolic blood pressure:model control group, ANJC group and enalapril control group, and ENJC groups (80%, 60%, 30%, 10% alternated respectively). SHR were given oral drug for 14 d accordingly. ALC-NIBP blood pressure monitoring system was employed to determine blood pressure before drug given and at 5 h, 6, 10, 14 d after drug administration, the blood pressure of 3, 7 d after drug application was also monitored. Result: After administration of 5 h, the rats of 80% ENJC showed significantly(P<0.05)reduced blood pressure, compared with the control group. On the 6 d of administration 80% and 60% ENJC showed significant antihypertensive effect, and the reduced DBP tended to be stable. After taking drug for 14 days, the hypotensive effect of 80% ENJC was most obvious. ENJC of 80% and 60% had significant effect of reducing heart rate. The function is proportional to the dose of ENJC. ANJC did not show the similar effect. Conclusion: Antihypertensive effect of ENJC is better than the original ANJC, with less dose, rapid onset and stonger and lasting effect.