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| UPLC同时测定银黄颗粒中4种黄酮类成分含量 | |
| 引用本文： | 乔东鸽,吉丽娜,何希荣,冯伟红,李东影. UPLC同时测定银黄颗粒中4种黄酮类成分含量[J]. 中国实验方剂学杂志, 2013, 19(15): 102-105 |
| 作者姓名： | 乔东鸽 吉丽娜 何希荣 冯伟红 李东影 |
| 作者单位： | 1. 河南省中医院,郑州,450002 2. 中国中医科学院中药研究所,北京 100700;中药质量控制技术国家工程实验室,北京 100700 3. 中国中医科学院中药研究所,北京,100700 4. 中药质量控制技术国家工程实验室,北京 100700;天津中医药大学,天津300193 |
| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09301-005,2009ZX09308-003) |
| 摘 要： | 目的:建立银黄颗粒中黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的UPLC含量测定方法.方法:采用ACQUITY UPLC H-Class超高效液相色谱仪,ACQUITY UPLC BEH C18色谱柱(2.1 mm× 50 mm,1.7 μm);流动相甲醇-0.2％磷酸水溶液,梯度洗脱,流速1 mL· min-1,检测波长274 nm,柱温30℃.结果:黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的线性范围分别为1.09 ～21.8,0.340～6.80,0.116 ～2.32,0.102～2.04 μg,加样回收率分别为100.8％(RSD 0.32％),97.1％ (RSD 0.95％),101.5％ (RSD 1.6％),98.7％(RSD 0.059％).结论:该方法简便、快速,分离效果好,可为全面控制银黄颗粒的质量提供参考. |
| 关 键 词： | 高效液相色谱 银黄颗粒 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 |
| 收稿时间： | 2013-03-29 |
Simultaneous Determination of Four Flavones in Yinhuang Granule by UPLC |
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| QIAO Dong-ge,JI Li-n,HE Xi-rong,FENG Wei-hong and LI Dong-ying. Simultaneous Determination of Four Flavones in Yinhuang Granule by UPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2013, 19(15): 102-105 | |
| Authors: | QIAO Dong-ge JI Li-n HE Xi-rong FENG Wei-hong LI Dong-ying |
| Affiliation: | Henan Province Hospital of Traditional Chinese Medicine, Zhengzhou 450002, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
| Abstract: | |
| Keywords: | UPLC Yinhuang granule baicalin wogonoside baicalein wogonin |
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