忍冬叶总黄酮的测定及其体外抗菌活性

目的:研究忍冬叶总黄酮的分离工艺及其对9种常见病原菌的体外抗菌活性。方法:以河南封丘忍冬叶为研究对象,以总黄酮为主要考察指标,通过大孔树脂柱色谱分离得到忍冬叶总黄酮,并对其进行最小抑菌浓度(MIC),最小杀菌浓度(MBC),抑菌圈活性等体外抗菌评价。结果:建立了忍冬叶总黄酮简便、有效的分离工艺,证实了忍冬叶总黄酮对9种供试病原菌具有较好的广谱抑菌性(MIC为1.95~62.5 g·L~(-1),MBC几乎均为125 g·L~(-1))。此外,当忍冬叶总黄酮部位同时含有较多绿原酸(4.2%)时,不仅得率高(10.5%),活性也更强。结论:忍冬叶总黄酮具有较好的广谱抗菌性,可以将其开发抗畜禽病原菌药物或者饲料添加剂,这也为忍冬叶资源的合理开发利用奠定了坚实的基础。

Determination of Total Flavonoids of Lonicera japonica Leaves and Their in Vitro Antibacterial Activity

Objective: To study the separation technology of total flavonoids from leaves of Lonicera japonica and investigate their antibacterial activity in vitro against nine common pathogenic bacteria. Method: The leaves of L. japonica from Henan Fengqiu were used, and the total flavonoids were obtained by macroporous resin column separation as the main investigation indexes. Then their antibacterial activity in vitro were evaluated by minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and inhibition zone. Result: A simple and convenient separation technology was established, proving the broad and better antibacterial activity against nine common pathogenic bacteria (MIC values were 1.95-62.5 g·L^-1, and MBC values were almost 125 g·L^-1). Meanwhile, when the total flavonoids fraction coexisted with plenty of chlorogenic acid (4.2%), its yields (10.5%) and activity were higher. Conclusion: The total flavonoids from the leaves of L. japonica could be developed as the antibacterial agent or feed supplement for livestock and poultry because of their broad and better antibacterial activity, and this study will lay the foundation for the rational development and utilization of the leaves of L. japonica.