健脾理气方对功能性消化不良大鼠胃肠运动功能及胃动素、胃泌素的影响

目的:研究健脾理气方对功能性消化不良(FD)大鼠胃肠运动功能及胃动素(MTL)、胃泌素(GAS)的影响,探讨该方调节胃肠动力的机制。方法:72只SPF级Wistar大鼠共分为6组,分别为正常组、模型组(空白组)、健脾理气方低、中、高剂量组及莫沙比利组,每组12只;健脾理气方高、中、低剂量组分别为80,48,16 g·kg-1;莫沙比利组予莫沙比利0.005 g·kg-1;空白对照组及模型组以等体积生理盐水ig,每天给药1次,从造模后第7天开始用药,持续给药14 d.观察健脾理气方对FD大鼠胃肠运动功能及MTL,GAS的影响。结果:健脾理气方各剂量组均可不程度地降低模型大鼠胃酚红残留率,升高模型大鼠小肠推进率(P<0.05或P<0.01),且高剂量组与莫沙比利组作用无显著性差异;与正常组相比,模型组大鼠血浆中MTL及GAS的含量明显降低,差异有显著性意义(P<0.05);不同剂量的健脾理气方均能不同程度增高大鼠血浆中MTL、GAS含量,差异有显著性意义(P<0.05);健脾理气方高剂量组与莫沙必利比较,差异无显著性意义。结论:健脾理气方可以改善脾虚气滞型FD大鼠的胃排空功能、促进小肠推进功能、提高血清胃动素及胃泌素水平等作用,这可能是其治疗FD的作用机制之一。

Effect of Decoction of Invigorating Spleen and Regulating Qi on Gastrointestinal Motility, Motilin and Gastrin in Rats with Functional Dyspepsia

Objective: To study the effect of the decoction of invigorating spleen and regulating Qi(DISRQ) on gastrointestinal motility, motilin(MTL),gastrin(GAS) in rats with functional dyspepsia(FD),exploring the mechanism of the DISRQ for adjusting gastrointestinal dynamics. Method: Seventy-two Wistar rats of SPF class were randomly divided into six groups i.e. normal group, model group,low dose of DISRQ group, middle dose of DISRQ group, high dose of DISRQ group and cispride group(n=12 each, male and female in half). The normal group and model group were given physiological saline intragastrically; while DISRQ groups were administrated with DISRQ at doses of 80,48,16 g ·kg^-1 respectively,and cisapride group was given cisapride of 0.005 g ·kg^-1. Investigation started from 7 days after modeling and continued for 14 successive days. Gastrointestinal Motility, MTL and GAS in each group were observed. Result: Different doses of DISRQ could improve the function of gastric emptying and promote intestinal propulsion of FD rats in different extent(P<0.05 or 0.01),and the effect of the high dosage showed no significant difference with those in the positive control group(cispride).Compared with those in normal group, the level of plasma MLT and GAS in the model group were remarkably decreased(P<0.05). Different doses of DISRQ could increase the content of MLT and GAS in different extent(P<0.05). There was no significant difference between high dose group of DISRQ and the cisapride group. Conclusion: The therapeutic effect of DISRQ in experimental FD rats might result from improving gastric emptying,promoting the small bowel peristalsis,enhancing the level of plasma MTL and GAS.