知母与盐知母对甲亢阴虚大鼠红细胞膜 Na+-K+-ATP酶影响的比较研究

目的:比较知母及盐知母对甲亢阴虚大鼠红细胞膜Na+-K+-ATP酶活力的影响,从能量代谢方面探讨知母盐炙后增强滋阴降火作用的炮制原理。方法:SD大鼠,雌雄各半,随机分为空白组,模型组,六味地黄丸组(2.2 g.kg-1),生知母、盐知母高剂量组(2 g.kg-1),生知母、盐知母低剂量组(1 g.kg-1);各组大鼠每天上午ig甲状腺素液2 mL(1.25 g.L-1),空白组ig生理盐水;下午各给药组ig相应的药液(10 mL.kg-1),空白组、模型组ig等体积生理盐水,3周后测定红细胞膜Na+-K+-ATP酶活性。结果:与模型组相比,生知母及盐知母各剂量组均能显著降低Na+-K+-ATP酶的活性(P<0.05),其中低剂量组盐知母作用优于生知母(P>0.05);高剂量组结果相反,即盐炙后作用降低(P>0.05),且高剂量组作用弱于低剂量组(P<0.05)。结论:知母及盐知母均具有滋肾阴清虚热的作用。

Influences of Anemarrhenae Rhizoma and One Processed with Salt-Water on Na+-K+-ATP Enzyme in Erythrocyte Membrane in Rats with Hyperthyroidism and Yin Deficiency

Objective: To compare the influences of Anemarrhenae Rhizoma and the one processed with salt-water on erythrocyte membrane Na⁺-K⁺-ATP enzyme activities in rats with hyperthyroidism and yin deficiency, and to clarify the nourishing yin principle by processed Anemarrhenae Rhizoma with salt water. Method: SD rats, half male and half female, were randomly divided into control group, model group, Liuwei Dihuang Wan group(2.2 g ·kg^-1), high dose Anemarrhenae Rhizoma group and the high dose group of Anemarrhenae Rhizoma processed with salt-water (2 g ·kg^-1), low dose Anemarrhenae Rhizoma group and accordingly the one processed with salt-water group(1 g ·kg^-1).Each rat was ig given thyroxine liquid 2 mL(1.25 g ·L^-1)every morning. Control group was ig given physiological saline. The treatment groups were ig given corresponding liquids(10 mL ·kg^-1)each afternoon. Control group and model group was ig given the same volume of saline. Continuous treatment for three weeks and then the activity of erythrocyte membrane Na⁺-K⁺-ATP enzyme was determined. Result: Anemarrhenae Rhizoma and the one processed with salt-water could significantly reduce Na⁺-K ⁺-ATP enzyme activity(P<0.05).In the low dose group, the effect for the one processed with salt-water was better,but the effect of the high dose groups appeared to be on the contrary. Conclusion: Anemarrhenae Rhizoma and the one processed with salt-water both can nourish kidney yin and clear asthenic fever.