小红参乙酸乙酯部位抗心肌缺血活性研究

目的:筛选出小红参抗心肌缺血主要活性部位,研究其抗心肌缺血活性。方法:通过去甲肾上腺素(norepinephrine,NE)诱导大鼠离体主动脉环收缩试验筛选小红参石油醚、乙酸乙酯、正丁醇、水部位,以主动脉环张力为观测指标,供试物浓度依次为1.5×10-3,5×10-3,1.5×10-2,5×10-2,5×10-1 g.L-1,确定小红参抗心肌缺血活性部位为乙酸乙酯部位,进一步进行此部位小鼠常压耐缺氧试验、家兔血小板聚集试验和抗垂体后叶素(pituitrin,Pit)致大鼠心肌缺血试验。结果:小红参乙酸乙酯舒张主动脉环效应明显强于其他3个部位,可能是小红参抗心肌缺血主要部位;小红参乙酸乙酯部位有一定抗小鼠常压耐缺氧作用,可显著抑制家兔血小板聚集活性,改善Pit致大鼠心肌缺血程度,明显增强大鼠心肌缺血模型血清SOD活性,降低MDA含量;1.17 g.kg-1小红参乙酸乙酯能改善Pit致大鼠心肌缺血程度,缺血后5 min T波变化百分率为8.6%±3.6%,明显增强大鼠心肌缺血模型血清SOD活性,降低MDA含量。结论:小红参乙酸乙酯部位可能是小红参抗心肌缺血的主要活性部位。

Effects of Ethyl Acetate Extraction of Rubia yunnanensis on Anti-myocardial Ischemia in Rats

Objective: To determine the main active extraction of Rubia yunnanensis and verified its anti-myocardial ischemia activity. Method: Norepinephrine (NE) induced contraction activity of rat arterial link with different concentration of extract of R. yunnanensis (1.5×10^-3, 5×10^-3, 1.5×10^-2, 5×10^-2, 5×10^-1 g ·L^-1) was compared. The main active extraction was ethyl acetate extraction. Then activity of the ethyl acetate extraction was verified by researches, including mouse hypoxia tolerance under normal pressure, platelet aggregation induced by adenosine disphosphate (ADP) and action on rat myocardial ischemia induced by pituitrin (Pit). Result: The results showed ethyl acetate extraction had significant anti-myocardial ischemia effects that included prolong mouse living time, inhibited ADP induce platelet aggregation, improved myocardial injury induced by Pit, also strengthened the activeness of SOD and reduced MDA content obviously. Conclusion: The ethyl acetate extraction could be the main active extraction of R.yunnanensis for anti-myocardial ischemia.