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颈总动脉套管法与颈外动脉结扎法建立局部低切应力动脉粥样硬化兔模型的比较
引用本文:周淑媛,王迎寒,巩仔鹏,阮从潇,阚晓溪,朱晓新.颈总动脉套管法与颈外动脉结扎法建立局部低切应力动脉粥样硬化兔模型的比较[J].中国实验方剂学杂志,2013,19(10):166-169.
作者姓名:周淑媛  王迎寒  巩仔鹏  阮从潇  阚晓溪  朱晓新
作者单位:1. 中国中医科学院中药研究所,北京,100700
2. 中国中医科学院中药研究所,北京 100700;承德医学院中药研究所,河北承德 067000
基金项目:国家直属科研院所技术开发研究专项资金(NCSTE-2007-JKZX-301);国家自然科学基金面上项目(30973901);重大新药创制综合性中药新药研究开发技术大平台项目(2009ZX09301-005-2-4);中国中医科学院自主选题项目(ZZ20090207)
摘    要:目的:通过对比颈总动脉套管法与颈外动脉结扎法建立动脉粥样硬化兔模型的试验研究,比较2种方法在制模时间,模型稳定性方面的差异.方法:雄性新西兰兔30只,随机分成空白组(n=6);套管组(n=8);结扎组(n=8);高脂组(n=8).所有试验兔在高脂喂养前、后分别检测血脂水平,于第8周处死动物,取套管近心端与结扎近心端血管行病理学检查.结果:高脂组饲养8周形成明显的高脂血症;与高脂组相比,单纯套管法颈动脉内膜脂质沉积较轻,但内膜明显增厚,内膜、中膜、外膜均出现不同程度的坏死;结扎法病变比套管法轻,但仍严重于正常组.结论:与单纯高脂饲养法相比,单纯套管法试验兔8周也可形成明显的动脉粥样性血管病变,套管近心端可形成局部低切应力模型,模型稳定性好.

关 键 词:动脉粥样硬化  动物模型  颈总动脉套管  颈总动脉结扎
收稿时间:2013/2/20 0:00:00

Comparison of Establishing Rabbits Carotid Atherosclerosis Model Induced by Low Shear Stress Using Carotid Artery Cannula and Carotid Artery Ligation
ZHOU Shu-yuan,WANG Ying-han,GONG Zi-peng,RUAN Cong-xiao,KAN Xiao-xi and ZHU Xiao-xin.Comparison of Establishing Rabbits Carotid Atherosclerosis Model Induced by Low Shear Stress Using Carotid Artery Cannula and Carotid Artery Ligation[J].China Journal of Experimental Traditional Medical Formulae,2013,19(10):166-169.
Authors:ZHOU Shu-yuan  WANG Ying-han  GONG Zi-peng  RUAN Cong-xiao  KAN Xiao-xi and ZHU Xiao-xin
Institution:Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, Chengde Medical University, Chengde 067000, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
Abstract:Objective: Study rabbits carotid atherosclerosis model test by comparing carotid artery cannula method with the external carotid artery ligation method, the differences of modeling time and model stability of two methods are obtained. Method: Thirty Male New Zealand rabbits were randomly divided into four groups which are control group (n=6); cannula group (n=8); ligation group (n=8); fat group (n=8). Lipid levels of all rabbits were measured before and after the high fat diet. After 8 weeks, all rabbits were sacrificed and pathological examinations were made by taking the cannula proximal end and ligation of the proximal end of the vascular. Result: High-fat group formed a significant hyperlipidemia after 8 weeks. Compared with the high-fat group, simply cannula carotid intimal lipid deposited lighter, but vascular intima thickness was obvious increasing, at the same time, intima, media and membrane were necrosis in varying degrees. The ligation lesions were lighter than the tube method, while it is still more serious than the normal group. Conclusion: Compared with the high-fat diet alone, using simple cannula technique, rabbits can also form atherosclerotic vascular lesions for 8 weeks, and sleeve proximal end would form a local low shear stress model which has good stability.
Keywords:atherosclerosis  animal models  carotid artery cannula  carotid artery ligation
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