白芍储藏一年芍药苷及二氧化硫残留变化

目的:探讨硫磺熏蒸白芍在储藏一年中其芍药苷及二氧化硫残留的变化,为解决白芍质量与安全的硫磺熏蒸相关性问题提供科学依据。方法:将白芍与硫磺熏蒸白芍药材及其饮片在相同条件下储藏一年,分别测定其芍药苷、二氧化硫残留量。结果:白芍硫磺熏蒸后芍药苷含量显著性降低;在切片及煎煮过程中芍药苷也有所下降;芍药苷含量最低为2.805%;符合2015年版《中国药典》中芍药苷含量不低于1.6%的要求;硫磺熏蒸会导致二氧化硫残留,以饱和硫磺熏蒸白芍,残留量最高为284.239 mg·kg~(-1),但低于二氧化硫残留量检查项目限度"二氧化硫残留量不得超过400 mg·kg~(-1)"的规定;且在切片、煎煮以及储藏过程中会损失大部分。结论:白芍经硫磺熏蒸后,芍药苷含量下降明显,且有二氧化硫残留;但软化、切片及煎煮过程中二氧化硫残留量大大降低,硫磺熏蒸技术现阶段可以保留。

Changes in Residues of Paeoniflorin and Sulfur Dioxide in Paeoniae Radix Alba

Objective: To study the changes in the residues of paeoniflorin and sulfur dioxide in Paeoniae Radix Alba after storage for one year, in order to provide the scientific basis for solving the sulfur fumigation problem in guaranteeing the quality and safety of herbal medicine paeoniflorin. Method: The residues of sulfur dioxide and paeoniflorin in Paeoniae Radix Alba and samples suffocated by sulfur after storage for one year under the same conditions were determined. Result: The contents of paeoniflorin in Paeoniae Radix Alba decreased significantly after being fumigated by sulfur and in the process of being sliced and decocted. The minimum content of paeoniflorin in the prepared slices of paeoniae Radix Alba was 2.805%, which was consistent with the requirement in Chinese Pharmacopoeia for no less than 1.6%. Sulfur dioxide residues were detected, with the maximum residue of 284.239 mg·kg^-1 in fumigated samples, which however was still lower than the requirement for no less than 400 mg·kg^-1 of sulfur dioxide residue, and would mostly lost during slicing, decoction and storage. Conclusion: Content of Paeoniflorin in Paeoniae Radix Alba decreased significantly after being fumigated by sulfur. Sulfur dioxide residues greatly reduced during softening, slicing and decoction, suggesting that the sulfur fumigation technique could be reserved at present.