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橘核中柠檬苦素类物质纯化工艺
引用本文:罗静,何中燕,裴瑾,康亚兰,刘维,陈翠平.橘核中柠檬苦素类物质纯化工艺[J].中国实验方剂学杂志,2014,20(20):13-16.
作者姓名:罗静  何中燕  裴瑾  康亚兰  刘维  陈翠平
作者单位:成都中医药大学, 成都 611137;成都中医药大学, 成都 611137;四川科伦药物研究院有限公司, 成都 610071;成都中医药大学, 成都 611137;成都中医药大学, 成都 611137;成都中医药大学, 成都 611137;成都中医药大学, 成都 611137
摘    要:目的:优选橘核中柠檬苦素类物质的大孔树脂纯化工艺。方法:通过静态吸附-洗脱试验综合考察16种不同厂家和极性大孔树脂对橘核中柠檬苦素类物质的纯化效果,筛选最佳树脂型号。通过单因素试验考察HPD-722型大孔树脂对柠檬苦素类物质的动态吸附-洗脱条件,包括清洗剂用量、上样液质量浓度、洗脱剂浓度及用量等。结果:HPD-722型大孔吸附树脂对柠檬苦素类物质的纯化效果最佳;最佳纯化工艺为上样液质量浓度在3.503 3~5.403 4 g·L-1,上样流速1~2 BV·h-1,样品溶液pH 6,水洗用量1 BV,80%乙醇6 BV为洗脱剂,洗脱流速2 BV·h-1,洗脱液pH 7;洗脱液中柠檬苦素类物质纯度82.82%,其中柠檬苦素22.53%,诺米林45.90%。结论:采用HPD-722型大孔吸附树脂纯化橘核中柠檬苦素类物质的工艺可行性较高,可有效提高柠檬苦素类物质纯度。

关 键 词:橘核  柠檬苦素类物质  大孔树脂  诺米林
收稿时间:3/4/2014 12:00:00 AM

Purification Technology of Limonoids in Citri Reticulatae Semen
LUO Jing,HE Zhong-yan,PEI Jin,KANG Ya-lan,LIU Wei and CHEN Cui-ping.Purification Technology of Limonoids in Citri Reticulatae Semen[J].China Journal of Experimental Traditional Medical Formulae,2014,20(20):13-16.
Authors:LUO Jing  HE Zhong-yan  PEI Jin  KANG Ya-lan  LIU Wei and CHEN Cui-ping
Institution:Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Sichuan Kelun Pharmaceutical Research Institute Co. Ltd, Chengdu 610071, China;Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
Abstract:Objective: To optimize purification process of limonoids in Citri Reticulatae Semen by macroporous resin. Method: Static adsorption and desorption tests were adopted to select optimal type from 16 kinds of macroporous resin with different manufacturers and polarities.Purification process was optimized by single factor tests. Result: HPD-722 macroporous resin was selected, optimal purification process was as following:the concentration of sample solution between 3.503 3-5.403 4 g·L-1, sampling speed of 1-2 BV·h-1, pH of sample solution 6, washed impurity by 1 BV of water and eluted by 6 BV of 80% ethanol with elution rate of 2 BV·h-1, eluent pH of 7;under these conditions, purity of limonoids was 82.82%, including limonin of 22.53% and nomilin of 45.90%. Conclusion: Limonoids can be purified by HPD-722 macroporous resin, this process can effectively improve the purity of limonoids.
Keywords:Citri Reticulatae Semen  limonoids  macroporous resin  nomilin
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