复方柴郁温胆汤及其拆方对抑郁模型 大鼠血清铜、锌含量的影响

目的:探讨复方柴郁温胆汤(柴胡、郁金、枳实、半夏、陈皮、竹茹、人参、茯苓、大枣、炙甘草)对抑郁模型大鼠血清铜、锌的影响。方法:健康雄性SD大鼠84只,随机分为7组。采用Katz刺激方法制备慢性轻度不可预见性应激(CUMS)抑郁模型。分别拆方为化痰组(半夏、陈皮、竹茹)、调气血组(柴胡、郁金、枳实)、养心脾组(人参、茯苓、大枣、炙甘草)。血清铜、锌采用原子吸收法检测。结果:采用孤养和CUMS刺激方法成功制备动物抑郁模型。模型组血清铜含量较正常组明显升高、血清锌含量较正常组明显降低;而柴郁温胆汤组较模型组比较均能够逆转上述改变;其中化痰组可以提高血清锌的水平、养心脾组可以降低血清铜的水平。结论:柴郁温胆汤具有良好的抗抑郁作用,同时能逆转大鼠行为学、体内微量元素锌和铜的改变;其中起主要作用的可能为化痰组和养心脾组药物。

Chaiyu Wendan Tang and its Component Influence the Level of Plasm Copper, Zinc to Depression Model of Chronic Unpredictable Mild Stress in Rats

Objective: To explore the effects of traditional Chinese drugs Chaiyu Wendan Tang (CYWDT) and its component on the serum copper, zinc in depressed rats. Method: Eighty-four male rats were divided into seven groups randomly. Model of depression was established by chronic unpredictable mild stress (CUMS) and living individually. The herbs in CYWDT was split into three categries, namely Huatan, Tiaoqixue and Yangxinpi. The level of serum copper and zinc was detected by atomic absorption method. Result: The model of depression was successfully established. Compared with control animals, the serum copper level in the model rat was increased significantly and the serum zinc level was decreased significantly. CYWDT could reverse all these changes. Huatan could significantly increase the level of serum zinc (P<0.05). Yangxinpi could significantly decrease the level of serum copper. Conclusion: CYWDT has a good effect for anti-depression. It can improve the behavior of the depressed rats, increase serum copper level and decrease serum zinc level significantly in depressed rats. Huatan and Yangxinpi are likely the major components of CYWDT.