| HPLC测定清金化痰汤中黄芩苷和栀子苷的含量 |
| |
| 引用本文： | 张培琴,苏松柏,张建玲.HPLC测定清金化痰汤中黄芩苷和栀子苷的含量[J].中国实验方剂学杂志,2011,17(15):69-71. |
| |
| 作者姓名： | 张培琴 苏松柏 张建玲 |
| |
| 作者单位： | 贵阳中医学院第一附属医院,贵阳,550001 |
| |
| 基金项目： | 2009年度中医药、民族医药科学技术研究课题(黔中医药发[2009]76) |
| |
| 摘 要： | 目的:建立清金化痰汤剂中黄芩苷和栀子苷的测定方法。方法:采用高效液相色谱法,色谱柱Agilent C18(4.6mm×250 mm,5μm),柱温30℃,测定黄芩苷流动相为甲醇-0.4%磷酸(47∶53),检测波长为280 nm,流速:0.8 mL.min-1,检测波长为280 nm;测定栀子苷流动相为甲醇-水(25∶75),检测波长为238 nm,流速1 mL.min-1。结果:传统清金化痰汤剂中黄芩苷含量为221.59 mg/剂,黄芩苷平均回收率为97.97%,栀子苷为335.73 mg/剂,栀子苷的平均回收率为98.36%。结论:此法简便快速,准确稳定,重现性好,成本低。
|
| 关 键 词： | 高效液相色谱 清金化痰汤 黄芩苷 栀子苷 |
| 收稿时间： | 2011/1/26 0:00:00 |
Determination of the Concentration of Baicalin and Geniposide in Qingjin Huatantang by HPLC |
| |
| ZHANG Pei-qin,SU Song-bai and ZHANG Jian-ling.Determination of the Concentration of Baicalin and Geniposide in Qingjin Huatantang by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2011,17(15):69-71. |
| |
| Authors: | ZHANG Pei-qin SU Song-bai and ZHANG Jian-ling |
| |
| Institution: | The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China;The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China;The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine, Guiyang 550001, China |
| |
| Abstract: | |
| |
| Keywords: | HPLC Qingjin Huatantang baicalin geniposide |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
| 点击此处可从《中国实验方剂学杂志》下载免费的PDF全文 |
|
