| 女贞子醇提物不同极性部位的体外抗氧化活性研究 |
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| 引用本文： | 姚卫峰,陈汀,张丽,丁安伟.女贞子醇提物不同极性部位的体外抗氧化活性研究[J].中国实验方剂学杂志,2011,17(22):138-140. |
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| 作者姓名： | 姚卫峰 陈汀 张丽 丁安伟 |
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| 作者单位： | 1. 南京中医药大学药学院,南京,210046
2. 南京中医药大学药学院,南京210046;江苏省方剂研究重点实验室,南京210046 |
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| 基金项目： | 国家自然科学基金项目(81001599); 教育部博士点新教师基金项目(20103237120010); 江苏省中医药管理局研究项目(LB09031); 南京中医药大学青年自然基金项目 (09XZR14) |
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| 摘 要： | 目的:研究女贞子乙醇提取物不同极性部位的体外抗氧化活性,旨在为女贞子抗氧化相关有效成分的分离提供依据。方法:在体外化学模拟的条件下,采用1,1-二苯基-2-苦肼基(DPPH)自由基法、邻苯三酚法测定其对DPPH自由基和对超氧阴离子自由基的清除能力。结果:女贞子醇提物的石油醚、乙酸乙酯、正丁醇3个萃取部位抗氧化结果显示,相同浓度下女贞子乙酸乙酯部位抗氧化效果最强,其次是正丁醇部位、石油醚部位。结论:测定了女贞子醇提物不同溶剂提取物的抗氧化活性,初步确定女贞子中抗氧化相关活性成分主要存在于女贞子醇提物的乙酸乙酯部位。
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| 关 键 词： | 女贞子 醇提物 抗氧化 二苯代苦味酰基自由基法 邻苯三酚法 |
| 收稿时间： | 5/5/2011 12:00:00 AM |
Study on Antioxidative Activities of Different Polarity Parts in Extracts with Ethanol from Ligustrum lucidum In vitro |
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| YAO Wei-feng,CHEN Ting,ZHANG Li and DING An-wei.Study on Antioxidative Activities of Different Polarity Parts in Extracts with Ethanol from Ligustrum lucidum In vitro[J].China Journal of Experimental Traditional Medical Formulae,2011,17(22):138-140. |
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| Authors: | YAO Wei-feng CHEN Ting ZHANG Li and DING An-wei |
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| Institution: | College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China;College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China; Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing 210046, China |
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| Abstract: | Objective: Antioxidative activities of different polarity parts in extracts with ethanol from Ligustrum lucidum were studied in vitro to supply the separating base of antioxidative components. Method: The antioxidant properties of different parts in ethanol extracts of L. lucidum were evaluated by different antioxidant tests, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, superoxide anion radical scavenging activity in vitro chemical simulated systems. Result: The antioxidant results of petroleum ether, ethyl acetate and 1-butanol parts in ethanol extract show that the antioxidant effect of ethyl acetate parts wasthe strongest. Conclusion: Antioxidative components could be justified existing in the ethyl acetate parts initially. |
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| Keywords: | Ligustrum lucidum ethanol extract antioxidative DPPH Pyrogallol |
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