阳和汤血清增强阿霉素对MCF-7肿瘤细胞抑制作用机制的研究

研究小鼠阳和汤血清增强阿霉素对MCF-7肿瘤细胞抑制作用的机制.方法　用1.08 g·mL-1阳和汤按小鼠体重20 mL·kg-连续ig7 d,末次给药1h采血,分离得含阳和汤1g·mL-1的血清(YHDS).用SMY培养细胞24 h,5-溴脱氧尿核苷(BrdU)掺入法检测细胞增殖,软琼脂克隆形成法检测细胞分化,Wes...

Influence of Complex Herba Lysmachiae Gramules on Experimental Kidney Stone and Its Diuretic,Spasmolytic and Anti-inflammatory Effects

Objective: To study the influence of complex herba lysmachiae gramules on the experimental kidney stone and its diuretic,spasmolyti and anti-inflammatory effects. Method: Fifty Wistar rats (n=10) were randomly divided into five group,the normal goups, the model group, the Shenshitong gramules group(3.3 g ·kg^-1), high complex herba lysmachiae gramules group(7.4 g ·kg^-1) and low complex herba lysmachiae gramules group(1.8 g ·kg^-1). The rat model of renal calcium oxalate stones was induced by administrated ethylene glycol and ammonia chloride, after 8 weeks modeled and administered, the incidence of kedney stone, calaium in kedneys, serum BUN,serum Cr were detected. The effects of complex herba lysmachiae gramules on ureter smooth muscle of rabbits in vitro,water-loaded diuretic in rats and anti-inflammatory was evaluated. Result: The number of renal stone formation, the contentration of calcum in kedneys, serum BUN and serum Cr were lower in high complex herba lysmachiae gramules group than that in model group, and lasted diuretic effects in water-loaded rat. High complex herba lysmachiae gramules group could obviously inhibit the average tension amplified on the ureter smooth muscle induced by Ach, and could markedly suppressed xylene-caused mouse ear swelling and granuloma by cotton ball. Conclusion: Complex herba lysmachiae gramules could inhibit calcium oxalate stone formation in experimental rats, and had diuresis,spasmolytic effects in vitro and anti-inflammatory actions.