胆胰宁对慢性胰腺炎大鼠血清及胰腺组织中超氧化物 歧化酶活性和丙二醛含量的影响

目的:通过观察中药胆胰宁(DYN)对慢性胰腺炎(CP)大鼠中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响,进而探讨该方药治疗慢性胰腺炎的机制。方法:用腹腔注射L-精氨酸的方法制造慢性胰腺炎大鼠模型。将大鼠分为正常对照组,模型对照组,小柴胡汤丸组,胆胰宁低、高剂量组,观察胆胰宁治疗4周后大鼠血清及胰腺组织中SOD活性和MDA含量。结果:胆胰宁低剂量组和高剂量组对提高大鼠血清及胰腺组织中SOD活性有统计学意义(P<0.01)。胆胰宁低剂量组和高剂量组对降低大鼠血清及胰腺MDA含量有统计学意义(P<0.01)。结论:胆胰宁能够有效地提高慢性胰腺炎大鼠血清及胰腺组织中SOD活性,降低其血清及胰腺组织MDA的含量,能够有效地对抗慢性胰腺炎形成过程中的胰腺组织损伤,从而对慢性胰腺炎具有显著的防治作用。

Effect of Danyining on Activity of Superoxide Dismutase and Level of Malonyldialdehyde in Serum and Pancreatic Tissue of Rat with Chronic Pancreatitis

Objective: To observe the influence of Danyining(DYN) on activity of superoxide dismutase (SOD) and the level of Malonyldialdehyde(MDA) in serum and pancreatic tissue to explore the mechanism of Danyining for treatment of chronic pancreatitis(CP). Method: The rat CP model was established by injection of L- Arg into abdomen. The model rats were randomLy assigned into five groups; normal control group, model group, Xiao-chai-hu-tang pilular group, DYN low-dose group and high-dose group. After four weeks of treatment, all rats were executed to measure the activity of SOD and level t of MDA in serum and pancreatic tissue. Statistics the experiment data. Result: DYN low-dose group and high-dose group could increase the activity of SOD (P<0.01)and reduce the level of MDA (P<0.01) in serum and pancreatic tissue. Conclusion: DYN has functions of increasing the activity of SOD and reducing the level of MDA in serum and pancreatic tissue, which indicates that DYN may prevent against injury of pancreatic tissue,and be beneficial to treatment of CP.