白花蛇舌草和半枝莲配伍微粉对移植性小鼠肝癌肿瘤组织Bcl-2,Bax表达的影响

目的:观察白花蛇舌草和半枝莲微粉对移植性小鼠肝癌H22生长抑制作用及其对肿瘤组织中Bcl-2和Bax表达的影响。方法:建立移植性H22动物模型,并将模型动物随机分为模型对照、软坚口服液7.8 mL.kg-1、白花蛇舌草和半枝莲普通粉15.6 g.kg-1、白花蛇舌草和半枝莲微粉高、中、低剂量(15.6,11.7,7.8 g.kg-1)6组,给药10 d后处死模型小鼠分离肿瘤,检测肿瘤大小、称质量,计算肿瘤抑制率,并将肿瘤组织切片,免疫组化法检测Bcl-2和Bax基因。结果:白花蛇舌草和半枝莲微粉高剂量组肿瘤抑瘤率达47.55%,与软坚口服液结果相近;白花蛇舌草和半枝莲微粉高、中、低剂量可降低肿瘤组织中Bcl-2蛋白基因表达,高、中剂量可升高Bax蛋白基因表达;与白花蛇舌草和半枝莲普通粉组相比,白花蛇舌草和半枝莲微粉高剂量组Bcl-2蛋白基因表达明显降低,Bax蛋白基因表达明显升高。结论:白花蛇舌草和半枝莲微粉可抑制H22瘤体生长,且下调Bcl-2蛋白表达,上调Bax蛋白表达。

Effect of Combined Hedyotic diffusa and Scutellariae Barbatae Herba Superfine Powder on Expressions of Bcl-2 and Bax Proteins in Tumor Tissues of Transplanted Hepatocarcinoma in Mice

Objective: To observe the effect of combined Oldenlandia diffusa and Scutellariae Barbatae Herba superfine powder on tumor growth of transplanted hepatocarcinoma in mice and expressions of Bcl-2 and Bax proteins in tumor tissues. Method: Sixty healthy Kunming mice,transplanted by H22 hepatocarcinoma cells,were divided into 6 groups,including model group, Ruanjian oral liquid group, combined O. diffusa and Scutellariae Barbatae Herba common grinding powder group and three groups of combined O. diffusa and Scutellariae Barbatae Herba superfine powder in high dose,medium dose and low dose.The mice were executed after ten days of treatment and tumor tissues were excised.The size of tumor tissues were measured and tumor inhibition rate was calculated.And expression of Bcl-2 and Bax was detected by using immunohistochemical method. Result: The tumor inhibition rate of Combined O. diffusa and Scutellariae Barbatae Herba superfine powder group in high dose was 47.55%,which was nearly same as Ruanjian Oral Liquid group.Compared with the model group, expression of Bcl-2 protein was decreased in the groups of Combined O. diffusa and Scutellariae Barbatae Herba superfine powder in high,medium and low dose; expression of Bax protein was increased in the groups of combined O.diffusa and Scutellariae Barbatae Herba superfine powder in high and medium dose. Compared with common grinding powder groupvexpression of Bcl-2 protein was increased and the expression of Bax Protein was decreased in the high group of Combined O.diffusa and Scutellariae Barbatae Herba superfine powder. Conclusion: Combined O. diffusa and Scutellariae Barbatae Herba superfine powder can inhibit the growth of tumor of H22,reduce the expression of Bcl-2 protein and increase the expression of Bax, which is worth further studying in antitumor action.