江西省锐尖山香圆亲缘关系与群体结构的ISSR分析

目的:利用简单序列重复区间扩增多态性(ISSR)分子标记技术对江西省锐尖山香圆进行亲缘关系和遗传结构分析,为该药材资源的保护和利用提供理论依据。方法:采集江西省4个县6个采样地的22份锐尖山香圆叶片样本,利用试剂盒法提取基因组DNA。利用64条通用ISSR分子标记引物进行聚合酶链式反应(PCR)扩增,运用聚丙烯酰胺凝胶电泳(PAGE)方法检测条带。选择NTsys 2. 10e软件,采用非加权配对算术平均法(UPGMA)计算遗传相似系数并聚类分析。利用Structure 2. 1软件分析群体遗传结构。结果:有48条ISSR引物扩增后获得了产物,多态性条带百分率处于45. 45%～100%。UPGMA聚类分析表明4个县的锐尖山香圆资源不能按照行政区域划分分别聚为一类,群体遗传结构分析表明22份锐尖山香圆群体可以划分为3个类群。结论:江西省锐尖山香圆群体间存在着基因交流,会影响该药材不同地理来源种质资源的遗传结构组成。

ISSR Analysis of Genetic Relationship and Population Structure of Turpinia arguta Fresh Leaves in Jiangxi Province

Objective To explore genetic relationship and population structure of Turpinia arguta in six locations of Jiangxi province by inter-simple sequence repeat (ISSR) molecular marker technique, and to provide theoretical basis for the protection and utilization of this medicinal material resource.Method A total of 22 samples from six locations in four counties in Jiangxi province were collected, and genomic DNA was extracted by kit method. Polymerase chain reaction (PCR) amplification was performed using sixty-four universal ISSR molecular marker primers, and the products were detected with polyacrylamide gel electrophoresis (PAGE). NTsys 2.10e software was selected to calculate the genetic similarity coefficient by unweighted pair group method with arithmetic mean (UPGMA) and cluster analysis. Population genetic structure was analyzed by Structure 2.1 software.Result A total of forty-eight ISSR primers were amplified to obtain the product, the percent of polymorphic bands ranged from 45.45% to 100%. UPGMA cluster analysis showed that these plant individuals could not be clustered according to their respective executive locations. Analysis of population genetic structure showed that 22 samples of T. arguta could be divided into three populations.Conclusion There is gene exchange among the populations of T. arguta in Jiangxi province, and it can affect the genetic structure of germplasm resources from different geographical sources.