全蝎蛋白药效组分对Bel7402肿瘤细胞凋亡的影响

目的:研究全蝎(Scorpio)蛋白药效组分对Bel7402肿瘤细胞凋亡的影响,为建立全蝎与临床疗效对应的生物效应质量评价方法奠定基础。方法:蛋白质提取和冷冻干燥的方法获得全蝎蛋白药效组分,采用改良MTT法、流式细胞法研究全蝎蛋白药效组分对Bel7402细胞毒和细胞周期抑制的作用。结果:抑制癌细胞生长的浓度大于或等于37 g.L-1,剂量与效应呈正比,相关系数为0.912 5,IC50为241 g.L-1,显效时间0～48 h;全蝎蛋白药效组分浓度大于或等于9.25 g.L-1时,能显著提高Bel7402细胞的凋亡率。结论:全蝎蛋白药效组分具有促进Bel7402细胞凋亡、抑制增殖的作用,其生物效应指标为:9.25～175 g.L-1,可作为全蝎生物效应质量评价的指标之一。

Inhibitory Effects of Expression of Protein Active Composition of Scorpio on Bel7402 Cells in vitro

Objective: To investigate the effects of protein active composition of Scorpio on inhibition of proliferation and on apoptosis in Bel7402 cells, and to supply the data on establishing the quality evaluation method of biological effect guided by the clinical effect of Scorpio. Method: Bel7402 cells were examined by trypan blue exclusion. The proliferation of cells was determined by improving MTT assay. Flow cytometry was performed to analyze cell apoptosis with propidium iodide(PI). Result: When the concentration of protein active composition of Scorpio exceeded or equaled 37 g ·L^-1, the coefficient correlation of the growth inhibiting curve to Bel7402 cells was 0.912 5, and IC₅₀is 241 g ·L^-1. The excellence time was 0 to 48 hours. When the concentration of protein active composition of Scorpio exceeded or equaled 9.25 g ·L^-1, the apoptosis ratio of Bel7402 cells was raised significantly. Conclusion: The protein active composition of Scorpio might promote the apoptosis of Bel7402 cells and restrain the proliferation of them. The antitumor biological effect of the protein active composition of Scorpio was 9.25-175 g ·L^-1. The protein active composition may be one of the indexes for quality evaluation of biological effect of Scorpio.