硝菔通结方对功能性便秘大鼠结肠组织中VIP及AQP3表达的影响

目的:探讨硝菔通结方对功能性便秘大鼠结肠组织中血管活性肠肽(VIP),水通道蛋白-3(AQP3)表达的影响。方法:成年SD雄性大鼠50只,随机分为正常组、模型组、硝菔通结方高、中、低剂量组(380,190,95 g·kg-1),每组10只。采用复方地芬诺酯(15 mg·kg-1)ig,建立SD大鼠功能性便秘模型,造模成功后各治疗组分别给予不同剂量硝菔通结方ig 3周。观察各组大鼠首粒黑便的排出时间、粪便干湿重变化,免疫组化检测大鼠结肠组织中VIP及AQP3的表达,RT-q PCR进一步检测结肠组织中VIP,AQP3 mRNA的表达水平。结果:与正常组比较,模型组大鼠粪便量少、质地干硬,首粒黑便排出时间明显延长,大便含水率减少;结肠组织中VIP表达降低,AQP3的表达升高,均具有统计学意义(P0.05,P0.01)。与模型组比较,硝菔通结方各剂量组大鼠粪便量增加,质地稀软,首粒黑便时间缩短,大便含水率增多;结肠组织中VIP mRNA表达明显升高,AQP3 mRNA的表达降低,均具有统计学意义(P0.05,P0.01)。结论:大鼠功能性便秘的发生可能与结肠组织中VIP及AQP3的异常表达有关,硝菔通结方治疗功能性便秘的机制可能是通过调节结肠组织中VIP及AQP3的表达来实现。

Effect of Xiaofu Tongjie Fang on Expression of VIP and AQP3 in Colon Tissue of Rats with Functional Constipation

Objective: To explore the effect of Xiaofu Tongjie Fang (XFTJF) on expressions of vasoactive intestinal peptide (VIP) and aquaporin 3 (AQP3) in colon tissue of rats with functional constipation. Method: Fifty adult male SD rats were randomly divided into the control group, the model group, the high-, medium-and low-dose XFTJF groups (380, 190, 95 g·kg^-1) of 10 rats in each group. The functional constipation model was induced by using compound diphenoxylate tablets (5 mg·kg^-1) orally. The rats received intragastric administration of XFTJF for 3 weeks. The general performance, the time to first black stool and fecal water content were observed. The expressions of VIP and AQP3 in rat colon tissue were detected by using immunohistochemical method. The mRNA expression levels of VIP and AQP3 in colon tissue were further detected by using RT-qPCR. Result: Compared with the control group, the time to first black stool significantly prolonged, fecal water content reduced, VIP mRNA level decreased, and AQP3 mRNA level increased in the model group (P <0.05, P <0.01). Compared with the model group, the time to first black stool shortened, fecal water content increased, VIP mRNA level increased, and AQP3 mRNA level decreased in all XFTJF groups (P <0.05, P <0.01). Conclusion: The occurrence of functional constipation in rats is probably related to the abnormal expressions of VIP and AQP3 in colon tissue. The mechanism of XFTJF in the treatment of functional constipation may be realized through regulating the expressions of VIP and AQP3 in colonic tissue.