下瘀血汤抗猪血清免疫性肝纤维化方证相关的药效学研究

目的：研究下瘀血汤抗大鼠猪血清肝纤维化方-证相关的药效学机制。方法：猪血清腹腔注射复制肝纤维化模型,造模8周后继续造模的同时以下瘀血汤、茵陈蒿汤、黄芪汤、二至丸和小柴胡汤进行干预治疗。测定大鼠血清ALT、AST、ALP和GGT活性,Alb、TP和TBil含量;Jamall法测定肝组织羟脯氨酸（Hyp）含量。结果：与正常组比较,模型组大鼠血清ALT和AST活性增高、Alb和TP含量显著下降（P〈0.05）,ALP和GGT活性及TBiL含量虽有升高但无显著性差异（P〉0.05）;与模型组比较,下瘀血汤、茵陈蒿汤和小柴胡汤组大鼠血清肝功能有一定的改善,以下瘀血汤组为最佳;与正常组比较,模型组大鼠肝组织Hyp含量显著增加（P〈0.05）;与模型组比较,下瘀血汤组大鼠肝组织Hyp含量显著降低（P〈0.05）,其它各药物组无改善作用（P〉0.05）。结论：下瘀血汤可有效的抑制大鼠猪血清免疫性肝纤维化,提示本模型病理阶段的证候为瘀血阻络。

Relationship Between Traditional Medical Formulae and Syndrome Based Pharmacological Study of Xiayuxue Decoction against Porcine Serum Induced Liver Fibrosis in Rats

Objective:To study relationship between traditional medical formulae and syndrome of Xiayuxue Decotion against liver fibrosis in rats induced by porcine serum.Methods:Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum.10 rats(4 in normal group and 6 in model group)were sacrificed after 8 w of modelling.The rest rats were then devided into normal group,model group,Xiayuxue Decoction group,Yinchenhao Decoction group,Huangqi Dectoction group,Erzhi Pill Group and Xiaochaihu Decoction Group.Porcine serum was continuous given at the same time of administration.Rats were sacrificed and sample was obtained at the end of 12 w of modeling.ALT,AST,ALP and GGT activity,and Alb,TP and TBil content in rats were determined.Hyp content in rat liver tissue was assayed with hydrochloric acid hydrolyzing method,and histological changes with HE staining and collagen deposition with sirus red staining.Results:ALT and AST levels increased significantly(P<0.05),Alb and TP content decrease markedly(P<0.05).For the increased ALP,GGT and TBil levels there are no.Compared with model rats the improved liver function of rats in treatment groups is found especially in Xiayuxue Decoction.Compared with normal group,Hyp content of liver tissue in modeling rats increased significantly(P<0.01).Compared with model group,liver tissue Hyp content in rats of Xiayuxue Decoction group decreases significantly(P<0.05),Hyp content in Yinchenhao Decoction group also decreases to some extent without statistical significance(P>0.05).Conclusion:Xiayuxue Decoction effectively inhibits liver fibrosis in rats induced by porcine serum,which indicated that liver fibrosis induced by porcine serum in rats might be blood stasis congesting vessels.