黄芪葛根汤对实验性糖尿病及胰岛素抵抗的影响

目的:观察黄芪葛根汤对实验性糖尿病及胰岛素抵抗(IR)的影响。方法:建立链脲佐菌素(STZ)ip所致糖尿病小鼠模型,sc氢化可的松琥珀酸钠(HCSS)诱导的IR小鼠模型,以及采用给大鼠1次ip小剂量链脲佐菌素(STZ),并加饲高热量饮食(富含脂肪和蔗糖),制备2型糖尿病(T2DM)伴胰岛素抵抗(IR)大鼠模型,观察黄芪葛根汤对糖脂代谢以及IR的影响。结果:黄芪葛根汤降低糖尿病小鼠的空腹血糖(FBG),提高糖耐量(OGTT),改善小鼠对胰岛素的敏感性。黄芪葛根汤能显著降低2型糖尿病胰岛素抵抗大鼠的FBG,提高OGTT,降低高胰岛素水平,提高胰岛素耐量,增加胰岛素敏感指数(ISI)和降低IR指数(HOMA-IR)指数。同时,黄芪葛根汤还可调节血脂、下调血清中游离脂肪酸(FFA)的水平。结论:黄芪葛根汤通过改善糖脂代谢、提高胰岛素的敏感性,发挥其治疗糖尿病及胰岛素抵抗的作用。

Effect of Huangqi Gegen Decoction on Experimental Diabetes Mellitus and Insulin Resistance

Objective: To observe effect of Huangqi Gegen decoction on diabetes mellitus and insulin resistance experimentally with models of mouse and rat. Method: Diabetic mouse model was induced by intraperitoneal injection of streptozotocin (STZ,160 mg·kg^-1), insulin resistance mouse model was induced by hypodermical injection of hydrocortisone sodium succinate. Type 2 diabetes model with insulin resistance (T2DM-IR) of rat was induced by intraperitoneal injection of lower dosage of STZ (30 mg·kg^-1) with high fat diet. The effects of Huangqi Gegen decoction on glucose or lipid metabolism and insulin IR were investigated with these models. Result: Huangqi Gegen decoction obviously improved fasting blood glucose and glucose tolerance of diabetic mice induced by STZ,increased insulin sensitivity in insulin resistance mice,also it showed significant lower fasting blood glucose, improved sugar tolerance, lowered serum hyperinsulinism, increased insulin tolerance, improved ISI and lowered HOMA-IR index, and regulated blood fat, down regulated serum free fatty acids in T2DM-IR rats. Conclusion: Huangqi Gegen decoction can improve glycolipid metabolism, increase insulin sensitivity to cure T2DM-IR.