| 近红外光谱法快速检测玉竹酒基提取液中多糖的含量 |
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| 引用本文： | 樊玲玲,张雯雯,丛晓东,蔡宝昌,钱建华,方小民,彭美仙.近红外光谱法快速检测玉竹酒基提取液中多糖的含量[J].中国实验方剂学杂志,2013,19(20):80-83. |
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| 作者姓名： | 樊玲玲 张雯雯 丛晓东 蔡宝昌 钱建华 方小民 彭美仙 |
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| 作者单位： | 浙江中医药大学中药炮制技术研究中心, 杭州 310053;浙江省省级药学类工程实践教学基地, 杭州 310053;浙江中医药大学中药炮制技术研究中心, 杭州 310053;浙江省省级药学类工程实践教学基地, 杭州 310053;浙江中医药大学中药炮制技术研究中心, 杭州 310053;浙江省省级药学类工程实践教学基地, 杭州 310053;浙江中医药大学中药炮制技术研究中心, 杭州 310053;浙江致中和实业有限公司, 浙江 建德 311607;浙江致中和实业有限公司, 浙江 建德 311607;浙江致中和实业有限公司, 浙江 建德 311607 |
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| 基金项目： | 卫生部科学研究基金项目(WKJ2010-2-019); 浙江省中医药科学研究基金计划项目(2012ZB032) |
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| 摘 要： | 目的: 建立测定玉竹酒基提取液中多糖含量的近红外光谱(NIR)快速分析方法。方法: 采用苯酚-硫酸法测定73批玉竹酒基提取液中多糖含量,采用傅里叶变换近红外透射光谱技术采集玉竹酒基多糖提取液的近红外光谱,结合偏最小二乘法(PLS)建立多糖的定量校正模型。结果: 玉竹酒基提取液多糖校正模型校正集的相关系数(R)、内部交叉验证均方差(RMSECV)分别为0.970 3,0.771,经外部验证,预测值与实测值的相关系数为0.969 2,预测均方差(RMSEP)为0.624。结论: 该方法快速方便、准确可靠,可用于玉竹酒基提取液多糖含量的快速测定。
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| 关 键 词： | 在线近红外光谱 定量分析 多糖 快速检测 |
| 收稿时间： | 5/6/2013 12:00:00 AM |
Rapid Determination of the Polysaccharide Content in Polygonatum Wine Matrix Extract by Near Infrared Reflectance Spectroscopy |
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| FAN Ling-ling,ZHANG Wen-wen,CONG Xiao-dong,CAI Bao-chang,QIAN Jian-hu,FANG Xiao-min and PENG Mei-xian.Rapid Determination of the Polysaccharide Content in Polygonatum Wine Matrix Extract by Near Infrared Reflectance Spectroscopy[J].China Journal of Experimental Traditional Medical Formulae,2013,19(20):80-83. |
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| Authors: | FAN Ling-ling ZHANG Wen-wen CONG Xiao-dong CAI Bao-chang QIAN Jian-hu FANG Xiao-min and PENG Mei-xian |
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| Institution: | Zhejiang Chinese Medical University, Research Center of Traditional Chinese Medicine Processing Technology, Hangzhou 310053, China;Provincical Pharmaceutical Engineering Practice Teaching Base in Zhejiang, Hangzhou 310053, China;Zhejiang Chinese Medical University, Research Center of Traditional Chinese Medicine Processing Technology, Hangzhou 310053, China;Provincical Pharmaceutical Engineering Practice Teaching Base in Zhejiang, Hangzhou 310053, China;Zhejiang Chinese Medical University, Research Center of Traditional Chinese Medicine Processing Technology, Hangzhou 310053, China;Provincical Pharmaceutical Engineering Practice Teaching Base in Zhejiang, Hangzhou 310053, China;Zhejiang Chinese Medical University, Research Center of Traditional Chinese Medicine Processing Technology, Hangzhou 310053, China;Zhejiang Zhizhonghe Industrial Corporation Limited, Jiande 311607, China;Zhejiang Zhizhonghe Industrial Corporation Limited, Jiande 311607, China;Zhejiang Zhizhonghe Industrial Corporation Limited, Jiande 311607, China |
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| Abstract: | Objective: To establish a near infrared spectroscopy method for the determination of polysaccharides in Polygonatum wine matrix extract. Method: The polysaccharide was measured by phenol-sulfuric acid method and the near-infrared spectroscopy of the Polygonatum wine matrix extract was collected by Fourier transform near infrared spectroscopy technology. Partial least square (PLS) was utilized to build the polysaccharide quantitative calibration model. Result: The results show that correlation coefficient (R), internal cross-validation mean square error (RMSECV) were 0.970 3 and 0.771.External validation samples collected on-line were used to evaluate the quantitative models. The root mean square error of prediction (RMSEP) and the correlation coefficient of the true value and prediction were 0.624 and 0.969 2. Conclusion: The presented method is rapid, accurate and reliable, which can be used in practice to fast determine polysaccharide content in Polygonatum wine matrix extract. |
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| Keywords: | on-line near infrared spectroscopy quantitative analysis polysaccharides rapid detection |
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