不同生境对粗毛淫羊藿光合色素与次生代谢物质的影响分析

目的: 分析粗毛淫羊藿植物在不同生境中光合色素和次生代谢物质的含量差异,探讨生境对药用植物生长与代谢的影响程度。 方法: 依据光照度与草本重要值将分布区划分为4种生境,设置样方采样。采用UV,HPLC检测各生境下植株叶中的叶绿素(a, b)、类胡萝卜素与总黄酮、淫羊藿苷的含量,运用SPSS 17.0软件比较分析。 结果: 在林缘旷地生境中植株叶绿素a(1.90 mg·g^-1)、叶绿素b(0.66 mg·g^-1)和类胡萝卜素(0.43 mg·g^-1)的含量最低,淫羊藿苷(0.36%)、总黄酮(9.13%)含量最高,相关性分析显示这2类物质呈显著负相关(P<0.01)。而灌木林、杂草丛、河谷沟渠生境中植株的光合色素、次生代谢物含量差异不显著。 结论: 在林缘旷地强光胁迫生境中,粗毛淫羊藿植物叶中总叶绿素和类胡萝卜素合成下降,植物生长不良,但次生代谢物质积累增加,表明该植物有明显的逆境效应。

Effect of Different Habitats on Contents of Photosynthetic Pigments and Secondary Metabolites of Epimedium acuminatum

Objective: The contents of photosynthetic pigments and secondary metabolites were analyzed in different habitats to explore the influence of growth and metabolism of Epimedium acuminatum. Method: The quadrat was divided into four habitats according to the illuminance and herb important value. The contents of chlorophyll a, chlorophyll b, carotenoids, total flavonoids and icariin were determined by UV method and HPLC method of E. acuminatum leaves. The date were analyzed by the SPSS 17.0. Result: Chlorophyll a (1.90 mg·g^-1), chlorophyll b (0.66 mg·g^-1) and carotenoids (0.43 mg·g^-1) were the lowest. And the contents of icariin and total flavonoid were the highest in the forest edge wilderness. Correlation analysis showed that photosynthetic pigments and secondary metabolites had significant negative correlation (P<0.01). However, the contents of photosynthetic pigments and secondary metabolites in E. acuminatum leaves had no significant difference in the bushes habitat, weeds habitat and valley ditch habitat. Conclusion: The synthesis of chlorophyll and carotenoids declined, resulting in poor growth, but the accumulation of secondary metabolites increased in the forest edge wilderness. The above shows that the plant of E. acuminatum has an obvious tress-effects.