降压宝蓝片对自发性高血压大鼠左室肥厚的影响

目的:研究降压宝蓝片对自发性高血压大鼠(SHR)左心室肥厚的影响。方法:将12周龄雄性SHR随机分为SHR组(0.5%CMC-Na),降压宝蓝片组(0.6 g·kg-1),依那普利组(0.02 g·kg-1),另设同龄正常血压的Wistar-kyoto(WKY)大鼠10只为正常组(0.5%CMC-Na),ig给药,连续12周。大鼠尾动脉血压计测定大鼠尾动脉收缩压(SBP),舒张压(DBP),平均动脉压(MAP)。超声心动图分别测量左室后壁厚度(LVPWT),室间隔厚度(IVST),左室舒张末内径(LVEDD),左心室收缩末内径(LVESD)及RR间期,计算左心室短轴缩短率(LVFS),左心室收缩末期容积(LVESV),左心室舒张末期容积(LVEDV),射血分数(LVEF)和每搏量(SV)。取全心及左心室称重,计算心脏指数(CI)和左心室指数(LVMI)。左室心肌组织HE染色,病理图像分析系统进行心肌细胞横径(CTD)测量和形态学观察。结果:与正常组比较,SHR组SBP,DBP和MBP显著升高(P0.01),LVEDV,LVESV和SV均明显降低(P0.01),CI,LVMI和CTD明显增加(P0.01),LVST,LVPWT,LVFS,LVEF有所增加,但无统计学意义;与模型组比较,降压宝蓝片能显著降低LVFS,LVEF,CI,LVMI和CTD(P0.05),明显改善左室心肌组织病理学改变;对血压有一定的降压作用,但降压幅度明显低于依那普利,且与SHR对照组比较无显著性差异。结论:降压宝蓝片具有改善高血压心肌肥厚的作用。

Effect of Jiangya Baolan Tablets Against Left Ventricular Hypertrophy in Spontaneously Hypertensive Rats

Objective: To investigate the effect of Jiangya Baolan tablets against left ventricular hypertrophy (LVH) in spontaneously hypertensive rats (SHR). Method: The 12-week-old male SHRs were randomly divided to the SHR group (0.5% CMC), the enalapril group (0.6 g · kg^-1) and the Jiangya Baolan tablets group (0.02 g · kg^-1). In addition, 10 12-week-old normotensive Wistar Kyoto (WKY) rats were included into the normal group (0.5% CMC). All of them were orally administered with drugs for 12 weeks. The rat tail sphygmomanometer was used to measure the systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP) of rat caudal arteries. The echocardiography was used to measure the left ventricular posterior wall thickness (LVPWT), interventricular septal thickness (IVST), left ventricular end-diastolic diameter (LVEDD), left ventricular end-systolic diameter (LVESD), left ventricular fractional shortening (LVFS), left ventricular end-systolic volume (LVESV), left ventricular end-diastolic volume (LVEDV), ejection fraction (LVEF) and stroke volume (SV). The cardiac index (CI) and left ventricular mass index (LVMI) were calculated by weighting the whole heart and left ventricle. The pathological image analysis system was used to measure the cardiomyocytes transection diameter (CTD) and observe the myocardial histopathology by hematoxylin and eosin (HE) staining. Result: Compared with the normal group, the SHR control group showed significant increases in the systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP) (P <0.01), obvious decreases in LVEDV, LVESV and SV (P <0.01), remarkable rises in CI, LVMI and CTD (P <0.01) and slight growths in LVST, LVPWT, LVFS and LVEF, but with no statistical significance. Compared with the normal group, the Jiangya Baolan tablets group showed notable decreases in LVFS, LVEF, CI, LVMI and CTD (P <0.05) and significant amelioration in the pathological changes of left ventricular myocardium, but with a slight antihypertensive effect that was obviously lower than that of enalapril and no significant difference with the SHR group. Conclusion: Jiangya Baolan tablets has the effect in ameliorating LVH in SHR.