灵芝体外抑制猴免疫缺陷病毒作用的研究

目的:对灵芝孢子水提物(AEGLS)进行体外抗猴免疫缺陷病毒(SIV)活性分析并对其作用机制进行初步研究.方法:300 TCID50的SIV病毒稀释液和AEGLS稀释液同时加入到CEM × 174细胞(CEM T淋巴细胞和174 B淋巴细胞杂交细胞)中培养4d后计数合胞体数量并进行统计,研究AEGLS抗SIV活性.利用不同加药时间点实验、作用阶段分析实验、Western blot对其作用机制进行初步分析.结果:AEGLS具有良好的抗SIV活性,其半数抑制浓度IC50为(466.62 ±20.21) mg·L-1,并且其细胞毒性较低,半数细胞毒性浓度CC50为(3.13±0.11) g·L-1,选择指数为6.71.抗SIV机制研究显示AEGLS主要作用在SIV病毒感染的早期阶段,与抑制SIV病毒的吸附或者穿透细胞有关,Western blot实验显示AEGLS能降低SIV衣壳蛋白P27( SIV P27)的表达水平.结论:AEGLS具有良好的抗SIV作用,其主要作用在SIV病毒感染的早期阶段,与抑制SIV病毒的吸附或者穿透细胞有关,并且能降低SlY P27农壳蛋白的表达水平.

Study on Effects of Inhibition of Ganoderma lucidum on Simian Immunodeficiency Virus in vitro

Objective: This paper presents the effects of anti simian immunodeficiency virus(SIV) of the aqueous extract from Ganodorma lucidumspores(AEGLS)by using SIVmac and CEM×174 cells as a model in vitro, and the mechanism were preliminarily studied. Method: The inhibitory effects of AEGLS on SIV were observed when SIV and CEM×174 system were employed as the models,and the mechanism of action were preliminarily studied by using time course assay, effective stage analysis,Western blot. Result: AEGLS had a remarkable anti-SIV activity. The IC₅₀ (50% inhibitory concentration) of AEGLS was (466.62±20.21)mg·L^-1, and it was low cytotoxicity which its CC₅₀ (50% cellular cytotoxicity) was (3.13±0.11)g·L^-1 and selection index was 6.71.The results of anti-SIV mechanism showed AEGLS main acted on the early stage of SIV infection,and might contribute to affect the SIV attachment and/or penetration into the cells. The results of Western blot showed AEGLS could decrease SIV P27 capsid protein expression level. Conclusion: AEGLS had a remarkable anti-SIV activity and is main acted on the early stage of SIV infection,and might contribute to affect the SIV attachment and/or penetration into the cells,and could decrease SIV P27 capsid protein expression level.