壳聚糖絮凝澄清工艺与醇沉工艺纯化乙肝宁复方水提液的对比

目的:探索壳聚糖絮凝澄清工艺与醇沉工艺对乙肝宁复方水提液的影响规律。方法:以干膏得率、药液澄明度、糖类及3种有效成分含量,浸膏吸湿率为评价指标,对上述2种纯化工艺进行对比考察。结果:2种纯化工艺的干膏得率相近;絮凝澄清工艺浸膏水溶液澄明度及其糖类、芍药苷的转移率均高于醇沉工艺,但其丹酚酸B和丹皮酚转移率均低于醇沉工艺。絮凝澄清工艺浸膏的吸湿性低于醇沉工艺浸膏。结论:为进一步研究中药浸膏固体制剂吸湿性机制与合理选择适宜纯化工艺用于中药制剂生产提供实验依据。

Comparative on Purifying Aqueous Solution of Yiganning Compound by Chitosan Flocculation Clarification Technology and Alcohol Precipitation Technology

Objective: To explore influence discipline of chitosan flocculation clarification and alcohol precipitation for aqueous solution of Yiganning compound. Method: Sample was investigated by multi-indexes to evaluate difference of two purification methods for aqueous solution of Yiganning compound, such as yield of extracts, physic liquor clarity, the content of carbohydrate(polyose, monosaccharide, oligosaccharide) and three active component(peoniflorin,salvianolic acid B and paeonol), moisture absorption ratio. Result: Yield of extracts of ethanol precipitation samples was close to chitosan clarification samples.Physic liquor clarity,transfer rate of carbohydrate and paeoniflorin of ethanol precipitation samples was lower than chitosan clarification samples,while transfer rate of salvianolic acid B and paeonol of ethanol precipitation samples are higher than chitosan clarification samples.Extracts hygroscopicity of ethanol precipitation samples are higher than chitosan clarification samples. Conclusion: The result provided a feasible method for investigating purification process of herbal extract preparation, and could be used for purification preparation craft optimization of herbal praeparatum.