痛泻要方主要有效成分的多靶点网络构建与机制分析

目的:对痛泻要方主要有效成分构建多靶点网络和机制分析,以阐明痛泻要方多成分、多靶点、多通路治疗肠易激综合征的药理机制。方法:利用数据库筛选出肠易激综合征相关基因,结合文献与数据库获取痛泻要方的目标成分和靶点,通过注释、可视化和集成发现数据库(DAVID)和京都基因组百科全书(KEGG)对靶点基因进行功能注释和通路富集分析,利用网络数据集成、分析和可视化(Cytoscape)软件构建痛泻要方的成分、靶点、疾病通路的相关网络并进行分析。结果:筛选得到痛泻要方中25种主要有效成分,相关靶点37个。以此为基础构建了目标成分-有效靶点(C-T)网络和成分-靶点-通路(C-T-P)网络,获得重要成分12个,核心靶点11个,关键通路26条,功能涉及胃肠道血管平滑肌、内分泌调节和神经免疫。结论:痛泻要方不同有效成分作用于多靶点网络,通过炎症反应调节、肠道感觉功能调节、免疫反应调节、精神心理调节和肠道感染与菌群紊乱调节等多途径发挥协同作用。

Multi-target Network Construction and Mechanism Analysis of Main Effective Components of Tongxie Yaofang

Objective: A multi-target network and mechanism analysis of the main effective components of Tongxie Yaofang was carried out to illustrate the multi-component, multi-target and multi-pathway pharmacological mechanism of Tongxie Yaofang in treating irritable bowel syndrome. Method: Genes associated with irritable bowel syndrome were screened out through databases. Literatures and databases were retrieved to obtain the components and targets of Tongxie Yaofang. Functional annotation and pathway enrichment analysis of target genes were carried out by the Database for Annotation, Visualization and Integrated Discovery (DAVID) and Kyoto Encyclopedia of Genes and Genomes(KEGG) databases. The components, targets and disease pathways-related networks of Tongxie Yaofang were constructed and analyzed by Cytoscape software. Result: A total of 25 main active components of Tongxie Yaofang were screened out, involving 37 related targets. Based on this, the target component-effective target (C-T) network and the component-target-pathway (C-T-P) network were constructed, including 12 important components and 11 core targets and 26 crucial pathways. The functions of target genes involved gastrointestinal vascular smooth muscle, endocrine regulation and nerve immunity. Conclusion: Various effective components of Tongxie Yaofang act on multiple targets network, and then have a synergistic effect by multiple channels, including regulating inflammation, intestinal sensory function regulation, immune response, psychological adjustment and intestinal infection and flora disorder.