| 二苯乙烯苷对6-OHDA损伤的SH-SY5Y细胞外氨基酸, 细胞内钙离子浓度的影响 |
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| 引用本文： | 焦玥,李涛,马淑骅,欧阳竞峰,王毅,孙丹丹,王丹巧,梁嵘.二苯乙烯苷对6-OHDA损伤的SH-SY5Y细胞外氨基酸, 细胞内钙离子浓度的影响[J].中国实验方剂学杂志,2015,21(15):111-115. |
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| 作者姓名： | 焦玥 李涛 马淑骅 欧阳竞峰 王毅 孙丹丹 王丹巧 梁嵘 |
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| 作者单位： | 中国中医科学院 医学实验中心, 北京市中医药防治重大疾病基础研究重点实验室, 北京 100700,中国中医科学院 医学实验中心, 北京市中医药防治重大疾病基础研究重点实验室, 北京 100700,中国中医科学院 医学实验中心, 北京市中医药防治重大疾病基础研究重点实验室, 北京 100700,中国中医科学院 医学实验中心, 北京市中医药防治重大疾病基础研究重点实验室, 北京 100700,中国中医科学院 医学实验中心, 北京市中医药防治重大疾病基础研究重点实验室, 北京 100700,中国中医科学院 医学实验中心, 北京市中医药防治重大疾病基础研究重点实验室, 北京 100700,中国中医科学院 医学实验中心, 北京市中医药防治重大疾病基础研究重点实验室, 北京 100700,北京中医药大学 基础医学院, 北京 100029 |
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| 基金项目： | 国家自然科学基金项目(81274121);中国中医科学院自主选题研究项目(ZZ2012010) |
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| 摘 要： | 目的: 观察二苯乙烯苷(TSG)对6-羟基多巴胺(6-OHDA)损伤的SH-SY5Y细胞的保护作用并探讨相关机制。 方法: 以SH-SY5Y细胞为研究对象,以100 μmol ·L-1 6-OHDA建立SH-SY5Y细胞损伤模型,以浓度6.25,12.5,25,50,100,200 μmol ·L-1 TSG作用损伤的SH-SY5Y细胞,另设空白组,四甲基偶氮唑盐(MTT)法测定细胞存活率,观察不同浓度的TSG对其的保护作用,激光共聚焦显微镜检测细胞内钙离子浓度的变化,高效液相-荧光法检测上清液中兴奋性氨基酸谷氨酸(Glu),抑制性氨基酸γ-氨基丁酸(GABA)含量。 结果: 与空白组比较,模型组细胞存活率降低,游离钙明显增多(P<0.01),细胞外Glu及GABA浓度明显升高(P<0.01);与模型组比较,TSG预处理24 h可以改善6-OHDA造成的细胞存活率的下降,其中12.5 μmol ·L-1的细胞保护作用最为明显(P<0.05),明显降低内游离钙含量,明显降低细胞外Glu及GABA浓度(P<0.05,P<0.01),TSG对其有明显的抑制作用。 结论: TSG可能通过减轻钙超载及兴奋性毒性对6-OHDA损伤的SH-SY5Y细胞起到保护作用。
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| 关 键 词： | 二苯乙烯苷 6-羟基多巴胺 钙离子 氨基酸 |
| 收稿时间： | 3/6/2015 12:00:00 AM |
Effect of Tetrahydroxystilbene Glucoside on Concentrations of Extracellular Amino Acid and Intracellular Calcium Ions in SH-SY5Y Cells Damaged by 6-OHDA |
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| JIAO Yue,LI Tao,MA Shu-hu,OUYANG Jing-feng,WANG Yi,SUN Dan-dan,WANG Dan-qiao and LIANG Rong.Effect of Tetrahydroxystilbene Glucoside on Concentrations of Extracellular Amino Acid and Intracellular Calcium Ions in SH-SY5Y Cells Damaged by 6-OHDA[J].China Journal of Experimental Traditional Medical Formulae,2015,21(15):111-115. |
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| Authors: | JIAO Yue LI Tao MA Shu-hu OUYANG Jing-feng WANG Yi SUN Dan-dan WANG Dan-qiao and LIANG Rong |
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| Institution: | Beijing Key Laboratory of Basic Research on Traditional Chinese Medicine Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China,Beijing Key Laboratory of Basic Research on Traditional Chinese Medicine Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China,Beijing Key Laboratory of Basic Research on Traditional Chinese Medicine Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China,Beijing Key Laboratory of Basic Research on Traditional Chinese Medicine Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China,Beijing Key Laboratory of Basic Research on Traditional Chinese Medicine Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China,Beijing Key Laboratory of Basic Research on Traditional Chinese Medicine Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China,Beijing Key Laboratory of Basic Research on Traditional Chinese Medicine Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China and School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China |
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