育麟方对卵巢储备功能降低大鼠颗粒细胞分泌功能的影响

目的: 研究育麟方影响环磷酰胺致卵巢储备功能降低大鼠模型卵巢颗粒细胞的分泌功能的机制。 方法: 性成熟雌性SD大鼠参考文献制备卵巢储备功能降低大鼠模型,分为育麟方高、中、低剂量组,补佳乐组,模型组,正常组。正常组,模型组予蒸馏水20 mL ·kg^-1,补佳乐组予戊酸雌二醇片0.21 mg ·kg^-1,育麟方低、中、高组分别予育麟方生药15.2,30.4,60.8 g ·kg^-1 ig 21 d后,予动情前期处死,放射免疫法测定大鼠血清卵泡刺激素(FSH),黄体生成素(LH)水平及抗苗勒管激素(AMH)表达水平,光镜下观察卵巢组织形态学变化,卵泡数目及黄体数目。 结果: 与正常组比较,模型组FSH,LH含量明显升高(P<0.05),AMH含量明显降低(P<0.05),各级卵泡数目,黄体数目明显减少(P<0.05);与模型组比较,育麟方治疗组大鼠血清FSH,LH水平明显减少(P<0.05),育麟方中剂量组及补佳乐组血清AMH水平明显增高(P<0.05),育麟方中、高剂量组的各级卵泡及黄体数量明显增多(P<0.05)。 结论: 育麟方可能通过影响卵巢储备功能降低模型鼠的颗粒细胞内分泌功能而改善其卵巢储备功能。

Effects of Yulin Decoction on Secretion Function of Granulosa Cells of Ovarian Reserve Declined Rats

Objective: To investigate the effect of Yulin decoction on the secretion function of granulosa cells in cytoxan-induced (CTX) ovarian reserve declined rats. Method: The rats were randomly divided into 6 groups:the normal group (distilled water, 20 mL · kg^-1), the model group (distilled water, 20 mL · kg^-1), the progynova group (0.21 mg · kg^-1), the high-, medium-and low-dose Yulin decoction groups (15.2, 30.4, 60.8 g · kg^-1) of 10 rats each. The ovarian reserve declined rats model were induced by injecting CTX intraperitoneally. The corresponding medicines were intragastrically administered rats for 21 days. Histomorphology changes of ovaries, the numbers of follicle and corpus luteum were observed, serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), anti-mullerian hormone (AMH) were determined by radioimmunoassay. Result: Compared with the normal group, the levels of FSH and LH increased, AMH level decreased, the numbers of follicle and corpus luteum decreased in the model group (P<0.05). Compared with the model group, the levels of FSH and LH decreased in Yulin decoction groups, AMH level increased in the medium-dose Yulin decoction and progynova groups, the numbers of follicle and corpus luteum increased in the medium-and high-dose Yulin decoction groups (P<0.05). Conclusion: Yulin decoction could improve the ovarian reserve function by regulating the secretion function of granulosa cells of ovarian reserve declined rats.