杞菊地黄汤改善实验性大鼠视网膜变性的作用观察

目的:观察杞菊地黄汤对实验性视网膜变性大鼠的治疗作用。方法:将生后45 d的SD大鼠分为正常组、模型组和杞菊地黄汤组。中药组大鼠ig杞菊地黄汤8.3 g.kg-1(15 mL.kg-1),模型组ig等体积生理盐水。两组动物均于生后47 d开始ig,每天1次,直至动物处死。生后50 d以N-甲基-N-亚硝脲(N-methyl-N-nitrosourea,MNU)ip造成视网膜变性模型,预定时间行闪光视网膜电图(flash electroretinograms,FERG)检测,造模后2,5 d时分批处死动物,取右眼行病理切片,分别以TUNEL法检测视网膜外核层细胞的凋亡、HE染色检测视网膜外核层的厚度。结果:ig杞菊地黄汤8.3 g.kg-1组大鼠FERG消失时间较模型对照组明显延迟;造模后2 d,其外核层细胞凋亡指数(50.8±7.3)%低于同期模型组大鼠(81.1±9.7)%(P<0.01);造模后5 d,其视网膜外核层厚度(71±27)μm大于同期模型组大鼠(22±10)μm(P<0.01)。结论:杞菊地黄汤对MNU诱导的光感受器细胞凋亡有保护作用。

The Ameliorative Action of Qiju Dihuang Decoction for Experimental Retinal Degeneration Rats

Objective: To observe the ameliorative effect of Qiju Dihuang decoction for N-methyl-N-nitrosourea (MNU)-induced retinal degeneration rats. Method: Sprague Dawley (SD) rats were divided into three groups: the control group, the model group and the Qiju Dihuang decoction group. After intraperitonal injected by the compound MNU, all SD rats were turned into retinal degeneration models. Every rat was tested by flash electroretinograms (FERG) at scheduled time points. The photoreceptor cells apoptosis was marked with terminal deoxynucleotidyl transferase mediated dUTP-digoxigenin nick end labeling (TUNEL). The thickness of outer nuclear layer (ONL) was measured by HE coloration. Result: Compared with the model control group, the Qiju Dihuang decoction can obviously sustain the FERG pattern of rats for a long time, remarkably reduce the apoptotic indexes and add the thickness of ONL. Conclusion: The Qiju Dihuang decoction can protect MNU-induced apoptosis of photoreceptor cells.