| 胃肠安对人胃癌SGC-7901细胞TGF-β1诱导上皮间质转化作用 |
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| 引用本文： | 沈克平,王海永,胡兵,刘威.胃肠安对人胃癌SGC-7901细胞TGF-β1诱导上皮间质转化作用[J].中国实验方剂学杂志,2011,17(23):132-135. |
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| 作者姓名： | 沈克平 王海永 胡兵 刘威 |
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| 作者单位： | 上海中医药大学附属龙华医院肿瘤五科,上海,200032 |
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| 基金项目： | 国家自然科学基金项目(30873258),上海市浦东新区中医领军型人才项目(PWZ2008-20-104) |
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| 摘 要： | 目的:观察胃肠安对人胃癌SGC-7901细胞上皮间质转化的影响。方法:接种SGC-7901细胞贴壁后,按5μg.L-1加入转化生长因子β1(transforming growth factor-β1,TGF-β1)建立人胃癌SGC-7901细胞上皮间质转化模型。同时,胃肠安组按1 000 mg.L-1加入胃肠安药液,继培5 d后,显微镜观察细胞形态,Western blot检测上皮、间质标记基因E-cad,N-cad,vimentin蛋白与相关激酶蛋白激酶B(Protein kinase B,PKB或AKT)、转录因子Twist,Snail蛋白表达情况。结果:较空白组,加入TGF-β1后,SGC-7901细胞发生上皮间质转化,细胞形态拉长变窄且细胞间连接更疏松,上皮标记基因E-cad蛋白相对表达量降低0.241;间质标记基因N-cad,vimentin蛋白相对表达量分别升高0.225,0.371。较模型组,胃肠安作用后,SGC-7901细胞大部仍成卵石状不典型上皮细胞形态,仅少数细胞出现间质细胞形态,上皮标记基因E-cad蛋白相对表达量升高0.210;间质标记基因N-cad,vimentin蛋白相对表达量分别降低0.119,0.124;相关通路AKT表达及磷酸化,转录因子Twist,Snail蛋白相对表达量分别降低0.248,0.085,0.402,0.382。结论:胃肠安可抑制人胃癌SGC-7901细胞上皮间质转化,可能与抑制AKT表达及磷酸化,下调转录因子Twist,Snail蛋白相关。
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| 关 键 词： | 胃肠安 胃癌 上皮间质转化 信号转导 |
| 收稿时间： | 2011/5/25 0:00:00 |
Effects of Weichangan Decoction on TGF-β1 Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells |
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| SHEN Ke-ping,WANG Hai-yong,HU Bing and LIU Wei.Effects of Weichangan Decoction on TGF-β1 Induced Epithelial Mesenchymal Transition in Gastric Carcinoma SGC-7901 Cells[J].China Journal of Experimental Traditional Medical Formulae,2011,17(23):132-135. |
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| Authors: | SHEN Ke-ping WANG Hai-yong HU Bing and LIU Wei |
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| Institution: | The 5th Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China;The 5th Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China;The 5th Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China;The 5th Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 20032, China |
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| Abstract: | |
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| Keywords: | Weichangan decoction gastric cancer epithelial mesenchymal transition signal transduction |
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