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家兔静脉注射辣椒碱纳米乳及溶液的药动学比较研究
引用本文:张鹏威,苏文琴,张莉,徐贵华,刘明生. 家兔静脉注射辣椒碱纳米乳及溶液的药动学比较研究[J]. 中国实验方剂学杂志, 2010, 16(18): 106-108
作者姓名:张鹏威  苏文琴  张莉  徐贵华  刘明生
作者单位:1. 海南医学院药学院,海口,571101
2. 武警医学院药剂教研室,天津,300162
3. 罗田万密斋医院药剂科,广东,罗田,436600
摘    要:目的:建立HPLC-荧光法测定家兔血浆中辣椒碱含量,并比较家兔静脉注射辣椒碱纳米乳及辣椒碱溶液的药动学参数差异。方法:采用双周期交叉试验设计,6只家兔分别单剂量注射辣椒碱纳米乳及溶液,分别在给药后3,6,9,12,15,25,40,60,90,120 min时取血,以HPLC-荧光法测定血药浓度,并计算主要药动学参数。结果:辣椒碱在0.197~49.3 g.L-1(r=0.999 6)内线性良好,最低检测限为5.9 ng.mL-1,回收率为102.5%~107.6%,RSD5%。辣椒碱纳米乳及溶液的消除半衰期t1/2分别为(11.77±2.32)min和(9.53±2.19)min,AUC为(5 311±1 324.6)ng.mL-1.kg-1.min和(4 909.5±1 110.4)ng.mL-1.kg-1.min。结论:HPLC-荧光法简便、准确、灵敏,适用于辣椒碱的血药浓度测定。家兔静脉注射辣椒碱纳米乳及溶液的主要药动学参数比较无显著性差异。

关 键 词:辣椒碱  药代动力学  高效液相色谱荧光法  纳米乳
收稿时间:2010-06-08

Pharmacokinetcs in Rabbits after Intravenous Injection of Capsaicin Nanoemulsions and Soluion
ZHANG Peng-wei,SU Wen-qin,ZHANG Li,XU Gui-hua and LIU Ming-sheng. Pharmacokinetcs in Rabbits after Intravenous Injection of Capsaicin Nanoemulsions and Soluion[J]. China Journal of Experimental Traditional Medical Formulae, 2010, 16(18): 106-108
Authors:ZHANG Peng-wei  SU Wen-qin  ZHANG Li  XU Gui-hua  LIU Ming-sheng
Abstract:Objective: To develop an HPLC-FLU method for the determination of capsaicin in rabbit plasma and to study its pharmacokinetics. Method: Six rabbits were given capsaicin nanoemulsions and ethanol solution (1mg ·kg-1) via ear vein by double cycle crossover design, and after 3, 6, 9, 12, 15, 25, 40, 60 and 120 min, blood samples were collected. HPLC- FLU was established to determine the plasma concentration of capsaicin and the pharmacokinetic evaluation was carried out. Result: The linear range of capsaicin was 0.197-49.3 mg ·L-1(r=0.999 6) and the limit of detection of capsaicin was 5.9 ng ·mL-1, the average recovery was 102.5%~107.6% with RSD<5%. The t1/2 of capsaicin nanoemulsions and solution was (11.77±2.32) min and (9.53±2.19) min respectively, and the AUC was (5 311±1 324.6) ng ·mL-1 ·kg-1 ·min and (4 909.5±1 110.4) ng ·mL-1 ·kg-1 ·min respectively. Conclusion: This assay method is simple, precise and sensitive enough for the pharmacokinetic study of capsaicin. The pharmacokinectics parameters between capsaicin nanoemulsions and solution had no significant difference ( t test, P>0.05).
Keywords:capsaicin  pharmacokinetics  HPLC-FLU  nanoemulsions
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