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变应性鼻炎豚鼠血清IgE和IL-6,IL-17的变化及消风宣窍汤的调节作用
引用本文:李萌,汪受传,魏肖云.变应性鼻炎豚鼠血清IgE和IL-6,IL-17的变化及消风宣窍汤的调节作用[J].中国实验方剂学杂志,2014,20(6):164-167.
作者姓名:李萌  汪受传  魏肖云
作者单位:南京中医药大学, 南京 210029;南京中医药大学, 南京 210029;南京中医药大学, 南京 210029
基金项目:江苏高校优势学科建设工程项目(苏政办发[2011]6号)
摘    要:目的:探讨实验性变应性鼻炎(llergic rhinitis,AR)发病机制中白介素(IL)-6、IL-17和免疫球蛋白E(IgE)水平的变化及其意义,分析消风宣窍汤对这3种细胞因子的调节作用。方法:用卵蛋白(OVA)通过腹腔注射结合滴鼻的方式建立AR豚鼠模型,造模周期共21 d,将60只Hartley豚鼠随机分为正常组、模型组、消风宣窍汤高、中、低剂量组(42,21,10.5 g·kg-1)、氯雷他定组(1.34 mg·kg-1)。用被动皮肤过敏试验(PCA)检验豚鼠模型制备情况,ig治疗1周,ELISA法检测各组血清中免疫球蛋白(IgE)和IL-6,IL-17的水平。结果:与正常组比较,模型组IgE和IL-6,IL-17水平明显升高(P<0.01);与模型组比较,消风宣窍汤高、中剂量组IgE水平显著降低(P<0.01),IL-6,IL-17水平降低(P<0.05或P<0.01);低剂量组IgE水平降低(P<0.05);氯雷他定组IgE水平降低(P<0.05),IL-6,IL-17水平显著降低(P<0.01)。结论:消风宣窍汤降低AR的IgE水平的机制可能是通过降低IgE和IL-6,IL-17水平实现的,并且两者呈正相关关系,提示IL-6和IL-17在AR发病机制中有重要作用。

关 键 词:消风宣窍汤  变应性鼻炎  免疫球蛋白E  白介素6  白介素17
收稿时间:6/2/2013 12:00:00 AM

Regulative Effect of Xiaofeng Xuanqiao Decoction Serum IgE and IL-6,IL-17 in Allergic Rhinitis Guinea Pig
LI Meng,WANG Shou-chuan and WEI Xiao-yun.Regulative Effect of Xiaofeng Xuanqiao Decoction Serum IgE and IL-6,IL-17 in Allergic Rhinitis Guinea Pig[J].China Journal of Experimental Traditional Medical Formulae,2014,20(6):164-167.
Authors:LI Meng  WANG Shou-chuan and WEI Xiao-yun
Institution:Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China;Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China
Abstract:Objective: To investigate the role of immunoglobin E(IgE) and interleukin-6(IL-6),interleukin-17(IL-17) in the pathogenesis of allergic rhinitis(AR),and the regulative effect of Xiaofeng Xuanqiao decoction on the above 3 cytokines. Method: AR guinea pig model was established by intraperitoneal injection and intranasal way with ovalbumin (OVA),and modeling cycle is 21 days. Sixty guinea pigs were randomized into 6 groups:normal control group,AR group,high-dosage,middle-dosage,low-dosage Xiaofeng Xuanqiao decoction group(42,21,10.5 g·kg-1),and loratadine group(1.34 mg·kg-1). Passive cutaneous anaphylaxis(PCA) test was performed in all guinea pigs. Xiaofeng Xuanqiao decoction and loratadine were applied to intervene AR guinea pig. The serum levels of IgE and IL-6,IL-17 were determined by enzyme-linked immunosorbant assay(ELISA) method. Result: The expression levels of IgE and IL-6,IL-17 significantly increased compared with the normal group(P<0.01). Compared with that in AR model group,the serum level of IgE in high-dosage Xiaofeng Xuanqiao decoction group significantly was decreased(P<0.01),the levels of IL-6,IL-17 were decreased(P<0.05);the levels of IgE and IL-6,IL-17 in equivalent-dosage Xiaofeng Xuanqiao decoction group significantly were decreased(P<0.01);the level of IgE in low-dosage Xiaofeng Xuanqiao decoction group decreased(P<0.05),the levels of IL-6,IL-17 have no obvious changes;the level of IgE in loratadine group decreased(P<0.05),and the levels of IL-6,IL-17 significantly decreased(P<0.01). Conclusion: Xiaofeng Xuanqiao decoction down-regulate serum level of IgE through decreasing the levels of IL-6 and IL-17,and there is a positive correlation between them,suggesting that IL-6 and IL-17 are important factors in the mechanism of AR.
Keywords:Xiaofeng Xuanqiao decoction  allergic rhinitis  IgE  IL-6  IL-17
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