当归补血汤促进小鼠骨髓移植后造血组织重建的研究

目的:探讨当归补血汤对小鼠同基因骨髓移植(BMT)后骨髓造血组织重建的影响。方法:建立典型同基因BMT小鼠模型,随机分成BMT模型对照组和BMT+当归补血汤组,另取正常小鼠外周血及骨髓作空白对照。分别给以生理盐水、当归补血汤10g·kg^-1·d^-1灌胃治疗。于BMT后第1,11,22天观察外周血细胞、骨髓单个核细胞(BMMNC),镜下观察小鼠骨髓切片。结果:BMT+当归补血汤组第11,22天外周血白细胞、红细胞、血小板、BMMNC水平均显著高于BMT模型对照组(P<0.05或P<0.01),骨髓造血组织学情况也明显好于对照组。结论:当归补血汤有促进造血组织重建的作用。

Effect of Danggui Buxue Decoction on Promoting Function Reconstruction of Hematopoietic Tissue after Bone Marrow Transplantation

Objective:To investigate the effect of Danggui Buxue Decoction on function reconstruction of hematopoietic tissue in mice after bone marrow isotransplanttation(iso-BMT).Methods:The typical mouse model of iso-BMT was established.The model mice were randomly divided into BMT+Danggui Buxue Decoction treatment group(treated with Danggui Buxue Decoction by gastrogavage) and BMT model group(treated with normal saline by gastrogavage).PB and BM of normal mice were obtained as blank.The following parameters were observed in the day 1,11 and 22:peripheral blood cells,bone marrow mono-nuclear cells(BMMNC),and the observation of bone marrow under microscope.Results:The levels of peripheral WBC,RBC,platelet,BMMNC,and the observation of bone marrow by microscope at the day 11 and 22 in BMT+Danggui Buxue Decoction treatment group were all significantly better than that of BMT model group(P<0.05 or P<0.01).Conclusion:Danggui Buxue Decoction could improve the function reconstruction of hematopoietic tissue.