天舒胶囊对利血平致小鼠偏头痛的影响

目的:观察天舒胶囊对利血平致小鼠偏头痛模型的作用,并探讨其作用机制.方法:ICR小鼠背部皮下注射利血平0.1 mg·kg-1造模,共12 d,正常对照组注射0.9％氯化钠注射液,于造模第7天开始ig给予天舒胶囊,剂量分别为生药1.55,4.65 g·kg-1,连续6d,第12天测定小鼠痛阈值,次日摘眼球采血检测凝血时间,取脑部组织分别测定5羟色胺(5-HT)、去甲肾上腺素(NA)含量,阳性对照为佐米曲普坦片(0.66 mg·kg-1).结果:与模型组相比,天舒胶囊能明显提高小鼠痛阈值(P＜0.01),延长凝血时间(P＜0.01),提高脑部NA和5-HT含量(P ＜0.05,P＜0.01),与阳性药相比,天舒胶囊明显提高脑部5-HT的含量(P＜0.05).结论:天舒胶囊对利血平低化5-HT致小鼠偏头痛有明显的改善作用,其平衡神经递质释放、抑制偏头痛发作期血管过度扩张可能是作用机制之一.

Effect of Tianshu Capsule on Migarine Model Induced by Reserpine

Objective: To study the effect and mechanism of Tianshu capsule on the migraine's mice, which was composed of Gastrodiae Rhizoma and Ligustici Chuanxiong Rhizoma. Method: Model was building on ICR mice after subcutaneous injection of reserpine 0.1 mg·kg^-1·d^-1×12 d, control group mice were injected 0.9% sodium chloride injection, treatment were started on the seventh day of modeling for 6 consecutive days, dose were 1.55, 4.65 g·kg^-1. The pain threshold was tested on the twelve day, the clotting time was tested next day and concentration of serotonin (5-HT), noradrenaline(NA) in the brain.was detected. Zolmitriptan tablets were used as a positive durg. Result: Compared with model group, the tianshu capsule could increase the pain threshold(P<0.01), prolong the clotting time(P<0.01), increase the concentration of 5-HT and NA in brain(P<0.05,P<0.01). Compared with positive drug, the capsule could increase the concentration of 5-HT(P<0.05). Conclusion: The capsule can treat the migraine's mice by balancing release of 5-HT, inhibiting vascular dilatation of migraine attacks.