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不同乙醇提取工艺对山豆根急性毒性的影响
引用本文:孙蓉,李素君,李晓宇.不同乙醇提取工艺对山豆根急性毒性的影响[J].中国实验方剂学杂志,2010,16(17):193-196.
作者姓名:孙蓉  李素君  李晓宇
作者单位:1. 山东省中医药研究院,济南,250014
2. 山东中医药大学,齐南,250355
基金项目:国家重点基础研究发展计划(973)中医基础理论专项资助项目 (2009CB522802);山东省科技平台建设项目课题(2008GG2NS02021)
摘    要:目的:比较不同乙醇提取工艺对山豆根急性毒性的影响。方法:分别制备山豆根乙醇热回流和渗漉提取液,采用经典急性毒性试验方法进行急性毒性比较研究,记录其毒性症状谱、累积死亡数和小鼠体重变化,用Bliss法分别计算半数致死量(LD50)及其95%可信度。结果:小鼠口服山豆根乙醇热回流液和渗漉液的LD50和95%可信限分别是27.135(24.869~29.622 g·kg-1)和13.399(12.016~14.899 g·kg-1),分别相当于人日用量的316.6倍和156.3倍,主要毒性症状谱为多动、烦躁、间歇性抽搐、呼吸急促等,最后因呼吸衰竭而死亡,成活动物体重与空白对照组相比有差异,药后7 d差异消失。结论:山豆根乙醇渗漉液的毒性大于热回流液,说明乙醇提取温度和浓度是影响山豆根急性毒性结果表达的关键因素,尤以温度为显著。

关 键 词:山豆根  乙醇提取工艺  急性毒性
收稿时间:2010/8/23 0:00:00

Comparative Research on Acute Toxicity of Different Alcohol Extracted Crafts of Sophorae Tonkinensis Radix et Rhizome
SUN Rong,LI Su-Jun and LI Xiao-yu.Comparative Research on Acute Toxicity of Different Alcohol Extracted Crafts of Sophorae Tonkinensis Radix et Rhizome[J].China Journal of Experimental Traditional Medical Formulae,2010,16(17):193-196.
Authors:SUN Rong  LI Su-Jun and LI Xiao-yu
Institution:Shandong Academy of Traditional Chinese Medicine,Jinan 250014,China;Shandong University of Traditional Chinese Medicine, Jinan 250355,China;Shandong University of Traditional Chinese Medicine, Jinan 250355,China
Abstract:ObJective: To compare the influence of different alcohol extraction on the acute toxicity of Sophorae Tonkinensis Radix et Rhizome , caused by different extracted crafts. Method : Separately using percolate and reflux extracted methods to prepare the extracts of different processed products. The classical acute toxicity methods were used in this study, the acute toxical symptoms, signs, death time and accumulated death rate were recorded and the LD50were calculated by Bliss method. Result : The LD50 and its 95% confident limit of reflux and percolation extracts on mice were respectively 27.135(24.869-29.622)g ·kg-1 and 13.399(12.016-14.899)g ·kg-1, which are equal to 316.6 and 156.3 times of the daily dosage in clinical, the main acute toxical symptoms such as: hyperactivity, restlessness, intermittent convulsion, breathlessness and died of respiratory failure eventually. The body weight between survival and normal mice had obvious difference (P<0.05), this difference disappeared after 7 d administration. Conclusion : The acute toxicity of different alcohol extraction of Sophorae Tonkinensis Radix et Rhizome is: percolation extracts>reflux extracts, explaining the key factors are temperature and concentration, especially the temperature, that affect the acute toxicity result expression of different alcohol extraction.
Keywords:Sophorae Tonkinensis Radix et Rhizome  alcohol extracted craft  acute toxicity
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