一测多评法测定虎杖中虎杖苷、白藜芦醇、大黄素及大黄素甲醚的含量

目的: 在建立虎杖中4种成分同步测定方法的基础上,建立一测多评法的方法学考察模式,验证该方法在虎杖中应用的可行性和技术适应性。 方法: 以虎杖为研究对象,建立大黄素与虎杖苷、白藜芦醇、大黄素甲醚的相对校正因子,并用该校正因子进行虎杖苷、白藜芦醇、大黄素甲醚的含量计算,实现一测多评;同时采用外标法测定饮片中虎杖苷、白藜芦醇、大黄素及大黄素甲醚的含量,并比较计算值与实测值的差异。 结果: 11批虎杖饮片中3种成分含量的计算值与实测值的RSD<3%,表明两者无显著差异。 结论: 以虎杖苷、白藜芦醇、大黄素和大黄素甲醚同步测定的一测多评法控制虎杖饮片的质量是可行的、准确的。

Simultaneous Quantitative Analysis of Polygonin, Resveratrol, Archen and Emodin Monomethyl Ether in Polygoni Cuspidati Rhizoma et Radix by Multi-Components Assay and Single Marker

Objective: To establish a new method of quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four components in Polygoni Cuspidati Rhizoma et Radix. Method: A HPLC method was developed as QAMS to determine polygonin, resveratrol, archen and emodin monomethyl ether in Polygoni Cuspidati Rhizoma et Radix. The relative correction factor (RCF) of other three components was determined by HPLC using archen as the internal reference substance. Content of the four components in 11 batches of samples, collected from different areas, was determined by both external standard method and QAMS. The method was evaluated by comparison of the quantitative results between external standard method and QAMS. Result: RSD of QAMS method and external standard method was less than 3%, which indicated that no siginificant differences between the quantitative results of QAMS method and external standard method were observed. Conclusion: It is feasible and suitable to evaluate the quality of Polygoni Cuspidati Rhizoma et Radix by QAMS.